• β2-Microglobulin Turbidimetric Immunoassay Reagent Kit

    SKU: 51680

    The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.

    • INTRODUCTION

      β2-Microglobulin (β2-MG), is a small-molecule globulin produced by lymphocytes, platelets, and polymorphonuclear leukocytes, with a molecular weight of 11,800 and a single-chain polypeptide consisting of 99 amino acids. It is the β chain (light chain) portion of the human leukocyte antigen (HLA) on the cell surface. It contains a pair of disulfide bonds in the molecule and contains no sugar; similar to the structure of the immunoglobulin stable region. It is widely found in plasma, urine, cerebrospinal fluid, saliva, and colostrum. Normal human β2-microglobulin synthesis rate and release from the cell membrane are fairly constant. Β2-microglobulin can be freely filtered from the glomerulus, 99.9% is absorbed in the proximal tubules, and in renal tubular epithelial cells Decomposition and destruction; therefore, the emission of β2-microglobulin is very small under normal circumstances.

      β2-MG mainly reflects glomerular filtration dysfunction, urine β2-MG mainly reflects renal tubular reabsorption function impairment. It also can be used as a tumor marker for some people with blood cell cancers (multiple myeloma, lymphoma) to give information about their likely prognosis. The health reference of β2-MG is 0.8-2 mg/L in blood and 0.11-0.32 mg/L in urine.

    • DATA SHEET

    • ASSAY CHARACTERISTICS

      A. Sensitivity

      The sensitivity is defined as the lower limit of detection and is estimated as the mean of the blank sample plus three times the SD obtained from the blank sample. The sensitivity of β2-MG assay is 0.042 mg/L.

       

      B. Precision

      The precision of the β2-MG assay is < 10% CV. Two samples consisting of serum-based panels were assayed 20 times separately.

       

      Sample

      Mean β2-MG

      (mg/L)

      SD

      (mg/L)

      CV

      Panel 1

      1.41

      0.008

      0.58%

      Panel 2

      1.73

      0.022

      3.00%

       

      C. Linearity

      The β2-MG assay is linear between 0.2 mg/L to 20 mg/L.

             

      D. Interference

      No interference was detected with hemoglobin up to 5 g/L, conjugated bilirubin up to 300 mg/L, free bilirubin up to 300 mg/L, and up to 5g/L lipid emulsion.

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