Rapid Human C-reactive protein ELISA kit

C-reactive protein (CRP) is a circulating protein mainly secreted from the liver. This acute phase protein consists of five identical non-glycosylated subunits of 23 kDa, that give rise to a symmetrically arranged globular protein with molecular weight of approximately 120 kDa.1 It has long been recognized that CRP is closely related to immunology, inflammation and host defence; as a result it has been used as an inflammatory marker. However, the development of high-sensitivity CRP (hsCRP) ELISA had addressed its role in other clinical issues. There is accumulating evidence suggesting the important role that CRP plays in mediating cardiovascular diseases (CVD) and type 2 diabetes.2-4 Normally CRP is presenting only in a trace amount in circulation (<1 µg/ml)5,6 but can increase over 1,000-fold under acute inflammatory state. Individual with blood CRP levels <1 µg/ml, 1-3 µg/ml and >3 µg/ml is considered to have low, moderate and high risk, respectively, of CVD and myocardial infraction.7 Therefore, blood CRP level has become a promising measure of CVD risk.8,9

A. Sensitivity:

The lowest level of CRP that can be detected by this assay is 0.312 ng/ml.

 

B. Specificity:

The antibody pair used in this assay is specific to human CRP and does not cross-react with mouse and rat CRP, and other cytokine or hormone molecules.

 

C. Precision

Intra-assay CV: 4.3%.

Inter-assay CV: 5.9%.

 

D. Recovery

The recovery of the assay was determined by adding various amounts CRP to a sample. The measured concentration of the spiked sample in the assay was compared to the expected concentration. The average recovery was 98%.

 

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