SARS-CoV-2 Nucleocapsid Protein (NP), HRP Conjugated
SARS-CoV-2 NP is expressed in E.coli and affinity purified.
The purified NP is conjugated to horseradish peroxidase (HRP) and stabilized in buffer.
Recombinant antigen for research use or manufacturing only.
1mg/ml. 100µL per vial.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Antigenicity validated in patient serum samples via ELISA by coating SARS-CoV-2 NP as capture antigen and using NP-HRP as a detector.
Strong antigenic response even in 1000-fold diluted patient serum.
ELISA: 1:5000 dilution
Western Blotting: 1:2500-1:10000 dilution.
Immunohistochemistry/immunocytochemistry: 1:1000-1:5000 dilution.
Optimal working dilutions must be determined by the end user.
STORAGE AND STABILITY
The undiluted antibody is stable at 2-8°C for 12 months. Do not store in a diluted format. DO NOT FREEZE.