SARS-CoV-2 Spike protein (expressed in CHO cell)
  • SARS-CoV-2 Spike protein (expressed in CHO cell)

    SKU: 41A252

     

     

    ★ Download Datasheet PDF ★

     

     

    Origin:              Recombinant

    Source:            CHO cells

    Tag:                  His at C-terminus

    Cat. No.:           41A252

    Size:                 100 ug

    Purity:               >95%

    Endotoxin:        <5 EU/mg, determined by the LAL method

    • INTRODUCTION TO THE MOLECULE

      The spike (S) glycoprotein of SARS-CoV-2 is a large trimeric class I fusion protein that is metastable and difficult to produce recombinantly in large scale. Here, we used plasmid with six beneficial proline substitutions modification to generate HexaPro Spike protein with better stability. Using cryo-EM, the structure of HexaPro spike protein is confirmed to retain the prefusion spike confirmation. Since S protein mediates the interaction of SARS-CoV-2 to the host cell receptor (ACE2), a stabilized prefusion spike protein can be used for the development of serological diagnostics and vaccines for SARS-CoV-2.

    • PRODUCT INFORMATION

      The recombinant SARS-CoV-2 HexaPro Spike Protein comprises residues 1-1208 (GenBank: MN908947) with C-terminal His-tag. The apparent molecular mass of HexaPro S in SDS-PAGE is appriximately 180 kDa. The concentration of protein was determined by BCA.

    • SDS-PAGE Gel

      See image

    • BIOACTIVITY AND ANTIGENICITY

      Strong binding ability with antibodies against SARS-CoV-2 S protein (determined by ELISA). 

    • FORMULATION, RECONSTITUTION AND STORAGE

      Liquid in sterile PBS, pH 7.4. The HexaPro protein can be stored at 2℃ to 8℃ for short-term (<1 week), and at -20℃ to -80℃ for long term store. Avoid repeated freeze-thaw cycles.

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