SARS-CoV-2 Spike protein (expressed in CHO cell)
Source: CHO cells
Tag: His at C-terminus
Cat. No.: 41A252
Size: 100 ug
Endotoxin: <5 EU/mg, determined by the LAL method
INTRODUCTION TO THE MOLECULE
The spike (S) glycoprotein of SARS-CoV-2 is a large trimeric class I fusion protein that is metastable and difficult to produce recombinantly in large scale. Here, we used plasmid with six beneficial proline substitutions modification to generate HexaPro Spike protein with better stability. Using cryo-EM, the structure of HexaPro spike protein is confirmed to retain the prefusion spike confirmation. Since S protein mediates the interaction of SARS-CoV-2 to the host cell receptor (ACE2), a stabilized prefusion spike protein can be used for the development of serological diagnostics and vaccines for SARS-CoV-2.
The recombinant SARS-CoV-2 HexaPro Spike Protein comprises residues 1-1208 (GenBank: MN908947) with C-terminal His-tag. The apparent molecular mass of HexaPro S in SDS-PAGE is appriximately 180 kDa. The concentration of protein was determined by BCA.
BIOACTIVITY AND ANTIGENICITY
Strong binding ability with antibodies against SARS-CoV-2 S protein (determined by ELISA).
FORMULATION, RECONSTITUTION AND STORAGE
Liquid in sterile PBS, pH 7.4. The HexaPro protein can be stored at 2℃ to 8℃ for short-term (<1 week), and at -20℃ to -80℃ for long term store. Avoid repeated freeze-thaw cycles.