SARS-CoV-2 Spike Protein (expressed in CHO cell)

SARS-CoV-2 Spike Protein (expressed in CHO cell)

SKU: 41A252

★ Download Datasheet PDF ★

 

 

Origin:              Recombinant

Source:            CHO cells

Tag:                  His at C-terminus

Cat. No.:           41A252

Size:                 100 ug

Purity:               >95%

Endotoxin:        <5 EU/mg, determined by the LAL method

  • INTRODUCTION TO THE MOLECULE

    The spike (S) glycoprotein of SARS-CoV-2 is a large trimeric class I fusion protein that is metastable and difficult to produce recombinantly in large scale. Here, we used plasmid with six beneficial proline substitutions modification to generate HexaPro Spike protein with better stability. Using cryo-EM, the structure of HexaPro spike protein is confirmed to retain the prefusion spike confirmation. Since S protein mediates the interaction of SARS-CoV-2 to the host cell receptor (ACE2), a stabilized prefusion spike protein can be used for the development of serological diagnostics and vaccines for SARS-CoV-2.

  • PRODUCT INFORMATION

    The recombinant SARS-CoV-2 HexaPro Spike Protein comprises residues 1-1208 (GenBank: MN908947) with C-terminal His-tag. The apparent molecular mass of HexaPro S in SDS-PAGE is appriximately 180 kDa. The concentration of protein was determined by BCA.

  • BIOACTIVITY AND ANTIGENICITY

    Strong binding ability with antibodies against SARS-CoV-2 S protein (determined by ELISA). 

  • FORMULATION, RECONSTITUTION AND STORAGE

    Liquid in sterile PBS, pH 7.4. The HexaPro protein can be stored at 2℃ to 8℃ for short-term (<1 week), and at -20℃ to -80℃ for long term store. Avoid repeated freeze-thaw cycles.

$286.00Price

For bulk order (QTY: 5 or more), please contact us for quote