Human TEV Protease
Retinol binding protein 4(RBP4), originally known as a specific transport of retinol in blood, is also a novel inflammatory and insulin resistance marker. Serum levels of RBP4 increased in insulin resistant and diabetes. Studies both in human and animal suggested that serum levels of RBP4 plays a key role in the link between obesity, insulin resistant and diabetes. Animal experiments found that increased secretion of RBP4 might reduces insulin-dependent glucose uptake by muscle tissue by reducing the activity of PI(3)K (phosphoinositide 3-kinase), and increased hepatic glucose output by increasing the expression of the enzyme PEPCK. However, whether serum RBP4 could be a biomarker of type 2 diabetes risk still remain unclear.
Total 211 AA. Mw: 24.4 kDa (calculated). N-terminal His-tag and TEV cleavage site,
28 extra AA (highlighted).
AMINO ACID SEQUENCE
Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C. Avoid repeated freezing/thawing cycles.
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let thelyophilized pellet dissolve completely
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Lyophilized in 1 mg/mL in PBS.
ELISA and Western Blotting.
<0.01EU per 1µg of protein by LAL method.