Monoclonal Antibody Development
High-Specificity mAb Generation for Research, Diagnostics & Therapeutics
IMD has decades of experiences in generating highly-specific monoclonal antibodies using hybridoma technology, as evidenced by the successful development of a large number of mAbs for research, clinical diagnosis, and therapeutic applications. We know how to generate a mAb that is specific to the immunogens and that works for your intended application. Our state-of-the-art facilities, including GE Biacore 8K and Sartorius Biostat B, ensure superior quality and performance at every step.
Our Services
1
Design and Production of peptide antigens
Design and production of peptide antigens with BSA and KLH conjugation. Gene synthesis, cloning with codon optimization, and recombinant protein expression and purification form selected host cells (E.coli, yeast, insect or mammalian systems). Alternatively, customers can provide their own antigen for immunization and screening.
Approximate Duration: 4-6 weeks
2
Immunization & Titer Evaluation
immunization if BALB/c mice using our optimized in-house protocols. Typically requires 3-4 immunizations to achieve desired serum titers, with additional boost immunizations available if needed. Serum antibody titers are rigorously evaluated by ELISA to selected the best candidates for fusion.
Approximate Duration: 8-10 weeks
3
Fusion, Cloning & Hybridoma Expansion
Splenocytes from mice with the highest serum titers are fused with SP2/0 myeloma cells. Hybridoma supernatants are screened by ELISA to identify positive clones. 2–4 rounds of subcloning ensure clonality and stability. Customers may choose 2 hybridoma clones for further expansion based on titer data, and aliquots of supernatant can be provided for in-house testing.
Approximate Duration: 6 weeks
4
Isotyping, Characterization & Production
Antibody isotyping, affinity, and binding kinetics determined by ELISA and Biacore® systems (GE Biacore 8K). Production options include serum-free medium or mouse ascites fluid (1–2 mg). Purification using Protein-A/G beads or antigen-specific affinity columns. Bulk production and purification with fermentation capabilities (Sartorius Biostat B).
Approximate Duration: 8 weeks
5
Cryopreservation & Long-Term Storage
Cryopreservation of hybridoma cells to ensure long-term viability and security. Stable cell line maintenance for future production needs, providing you with reliable access to your mAb clones for ongoing research and development.