Polyclonal Antibody Development
Custom pAb Generation for Research, Diagnostics & Assay Development
IMD provides a comprehensive range of quality services for the production of polyclonal antibodies (pAbs) in rabbits, rats, and mice. From antigen design and peptide synthesis to animal immunization, serum collection, antibody purification, characterization, and labeling—we deliver custom antibodies with superior specificity, affinity, and assay utility for diverse applications including IHC, IF, FACS, ELISA, Western blotting, and immunoprecipitation.
Our Services
1
Antigen Design & Preparation
Synthesis and cloning of target genes with codon optimization into your desired expression vector. Protein expression and purification from selected host systems. Alternatively, design, synthesis, and conjugation of peptide antigens with carrier proteins (BSA or KLH). Customers may also provide their own antigens for immunization. Our in-depth knowledge of antigen-determining factors—including size, secondary structure, and immunogenicity—ensures the production of antibodies with exceptional specificity and affinity.
Approximate Duration: 4-6 weeks
2
Animal Immunization & Titer Evaluation
Flexible immunization options to suit your project needs:
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2 rabbits, 3 immunizations, 70 days
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2 rats, 3 immunizations, 70 days
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5 mice, 3 immunizations, 70 days
Serum antibody titers are rigorously evaluated to confirm strong immune responses before proceeding to collection.
Approximate Duration: 8-10 weeks
3
Bleeding & Serum Collection
High-yield recovery for downstream applications
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~100 mL serum from two rabbits
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~6–8 mL serum from two rats
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~5–10 mL serum from five mic.
Serum is carefully processed and stored to preserve antibody activity and ensure high-quality starting material for purification.
Approximate Duration: 2 weeks
4
Antibody Purification
Tailored purification strategies for your application.
Options include:
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Protein A/G purification for total IgG recovery
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Antigen affinity purification for monospecific, high-purity antibodies targeting discrete epitopes
Our expertise in custom peptide and monospecific peptide antibody generation ensures antibodies are purified to meet the demands of your specific assay or application.
Approximate Duration: 2-3 weeks
5
Antibody Labeling & Conjugation
Ready-to-use detection reagents for your assays.
Custom labeling services available for:
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Biotin
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Horseradish peroxidase (HRP)
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Alkaline phosphatase (AP)
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Fluorochromes and dyes
Labeled antibodies are validated to ensure retained specificity and functionality for your intended detection system.
Approximate Duration: 1-2 weeks