• α1-Microglobulin Turbidimetric Immunoassay Kit

    SKU: 51900

     

    ★ Download Datasheet PDF ★

    ★ Download MSDS ★

     

    Assay Range: 6 - 100 mg/L

     

    The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.

    • INTRODUCTION

      α1-Microglobulin (A1M), also known as Protein HC, is a type of small globular protein containing 167 amino acids, which can be found in bloodstream and extravascular tissues of all organs. It is produced mainly in the liver and is broken down in the kidney. This low molecular weight protein can be filtered by the glomerulus and reabsorbed and catabolized by the proximal tubular cells.

      The level of A1M is useful in screening for tubular abnormalities and detection of chronic asymptomatic renal tubular dysfunction. In healthy subjects, the concentration of A1M is < 30 mg/L in serum, or the ratio of A1M and creatinine in the urine is over 0.7 mg/mmol. Higher than this range is indicative of renal dysfuction, including acute kidney failure, glomerular disease and diabetic nephropathy?

      IMD A1M PETIA kit can accurately measure A1M in human serum, plasma and urine samples.

    • Packing Specifications

       

      Cat. No.

      Size

      Approximately tests

      51900-05

      R1: 15ml, R2: 5ml

      100

      51900-10

      R1: 30ml, R2: 10ml

      200

      51900-20

      R1: 60ml, R2: 20ml

      400

      51900-50

      R1: 150ml, R2: 50ml

      1000

      51900-100

      R1: 300ml, R2: 100ml

      2000

       

      For bulk order, please enquire

       

    • DATA SHEET

    • Principle of the assay

      This assay is a turbidimetric immunoassay for the quantitative measurement of A1M in human serum and plasma.  A standard or sample is added into a cuvette and mixed with the reaction buffer R1. After a short incubation, the test reagent R2, which is a suspension of microparticles coated with A1M antibodies, is added into the cuvette and mixed. The presence of A1M in the standard or sample causes the immune-particles to aggregate. The extent to which the microparticles aggregate is quantified by the amount of light scattering measured as absorbance by a chemistry analyzer. The concentration of A1M in unknown samples can be interpolated from a reference curve using the standards provided.

    • Materials for the assay

      Product           

      Catalog           Number

      Format

      Method

      Kit

      51900

      R1/R2

      (Dual Vial Liquid Stable       

      Dual Vial Liquid Stable,

      Immunoturbidimetric             

      Calibrator                 

      51900-S1

      5 x 1ml

       

      Control

      51900-C1

      2 x 1ml

       

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