α1-Microglobulin (A1M) Turbidimetric Immunoassay Kit
α1-Microglobulin (A1M), also known as Protein HC, is a type of small globular protein containing 167 amino acids, which can be found in bloodstream and extravascular tissues of all organs. It is produced mainly in the liver and is broken down in the kidney. This low molecular weight protein can be filtered by the glomerulus and reabsorbed and catabolized by the proximal tubular cells.
The level of A1M is useful in screening for tubular abnormalities and detection of chronic asymptomatic renal tubular dysfunction. In healthy subjects, the concentration of A1M is < 30 mg/L in serum, or the ratio of A1M and creatinine in the urine is over 0.7 mg/mmol. Higher than this range is indicative of renal dysfuction, including acute kidney failure, glomerular disease and diabetic nephropathy?
IMD A1M PETIA kit can accurately measure A1M in human serum, plasma and urine samples.
PRINCIPLE OF THE ASSAY
This assay is a turbidimetric immunoassay for the quantitative measurement of A1M in human serum and plasma. A standard or sample is added into a cuvette and mixed with the reaction buffer R1. After a short incubation, the test reagent R2, which is a suspension of microparticles coated with A1M antibodies, is added into the cuvette and mixed. The presence of A1M in the standard or sample causes the immune-particles to aggregate. The extent to which the microparticles aggregate is quantified by the amount of light scattering measured as absorbance by a chemistry analyzer. The concentration of A1M in unknown samples can be interpolated from a reference curve using the standards provided.
PERFORMANCE OF THE KIT
TYPICAL STANDARD CURVE
Absorbance (570 nm)
Assay procedures may vary depending on the automated chemistry analyzer to be used. A general example of assay procedures is stated as follow:
1. Dispense 150µl of R1 into a clean cuvette
2. Add 1.5µl of sample and incubate at 37˚C for 5 minutes
3. Further add 50µl of R2
4. Read change of absorbance at Main Wavelength 570 nm for 8 minutes after the addition of R2
5. Calculate the concentration of A1M in unknown sample by interpolation from a reference curve using the standards provided
R1: 15ml, R2: 5ml
R1: 30ml, R2: 10ml
R1: 60ml, R2: 20ml
R1: 150ml, R2: 50ml
R1: 300ml, R2: 100ml
For bulk order, please enquire