α1-Microglobulin Turbidimetric Immunoassay Reagent Kit
The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.
α1-Microglobulin (A1M), also known as Protein HC, is a kind of small globular protein, which can be found in blood plasma and extravascular tissues of all organs. It is produced in most cells of the body, but mainly in the liver. A1M functions as a kind of radical scavenger and reductase, which continuously remove free radicals and also oxidizing agents, mainly heme from the tissues. This can help protect cells and also tissues from damages.
A1M is subsequently transported to liver, where it will be broken down. However, in the case of proteinuria, A1M cannot be completely broken down in the kidney, which will result in the accumulation of A1M in the bloodstream. Therefore, A1M can be used as marker for proteinuria. The result is positive when A1M level exceeds 30 mg/L or the ratio of A1M (in milligrams) and creatinine (in millimoles) in the urine is over 0.7 mg/mmol
The sensitivity is defined as the lower limit of detection and is estimated as the mean of the blank sample plus three times the SD obtained from the blank sample. The sensitivity of A1M assay is 0.181mg/L.
The precision of the A1M assay is < 10% CV. Two samples consisting of serum based panels were assayed 20 times separately.
The A1M assay is linear between 6 mg/L to 100 mg/L.
No interference was detected with hemoglobin up to 5 g/L, conjugated bilirubin up to 300 mg/L, free bilirubin up to 300 mg/L, and up to 5g/L lipid emulsion.