β2-Microglobulin (B2M) Turbidimetric Immunoassay Kit
β2-Microglobulin(β2-MG), is a small-molecule globulin produced by lymphocytes, platelets, and polymorphonuclear leukocytes, with a molecular weight of 11,800 and a single-chain polypeptide consisting of 99 amino acids. It is the β chain (light chain) portion of the human leukocyte antigen (HLA) on the cell surface. It contains a pair of disulfide bonds in the molecule and contains no sugar; similar to the structure of the immunoglobulin stable region. It is widely found in plasma, urine, cerebrospinal fluid, saliva, and colostrum. Normal human β2-microglobulin synthesis rate and release from the cell membrane are fairly constant. Β2-microglobulin can be freely filtered from the glomerulus, 99.9% is absorbed in the proximal tubules, and in renal tubular epithelial cells Decomposition and destruction; therefore, the emission of β2-microglobulin is very small under normal circumstances.
β2-MG mainly reflects glomerular filtration dysfunction, urine β2-MG mainly reflects renal tubular reabsorption function impairment. It also can be used as a tumor marker for some people with blood cell cancers (multiple myeloma, ly -mphoma) to give information about their likely prognosis. The health refere- nce of β2-MG is 0.8-2 mg/L in blood and 0.11-0.32 mg/L in urine.
IMD developed β2-MG PETIA kit uses its unique antibodies and antigens, with accuracy and specificity fully validated using international standard
PRINCIPLE OF THE ASSAY
This assay is a turbidimetric immunoassay for the quantitative measurement of β2-MG in human serum and plasma. A standard or sample is added into a cuvette and mixed with the reaction buffer R1. After a short incubation, the test reagent R2, which is a suspension of microparticles coated with β2-MG antibodies, is added into the cuvette and mixed. The presence of β2-MG in the standard or sample causes the immune-particles to aggregate. The extent to which the microparticles aggregate is quantified by the amount of light scattering measured as absorbance by a chemistry analyzer. The concentration of β2-MG in unknown samples can be interpolated from a reference curve using the standards provided.
PERFORMANCE OF THE KIT
TYPICAL STANDARD CURVE
Absorbance (570 nm)
Assay procedures may vary depending on the automated chemistry analyzer to be used. A general example of assay procedures is stated as follow:
1. Dispense 180µl of R1 into a clean cuvette
2. Add 2µl of sample and incubate at 37˚C for 5 minutes
3. Further add 60µl of R2
4. Read change of absorbance at Main Wavelength 570 nm for 8 minutes after the addition of R2
5. Calculate the concentration of β2-Microglobulin in unknown sample by interpolation from a reference curve using the standards provided
R1: 15ml, R2: 5ml
R1: 30ml, R2: 10ml
R1: 60ml, R2: 20ml
R1: 150ml, R2: 50ml
R1: 300ml, R2: 100ml
For bulk order, please enquire