β2-Microglobulin (B2M) Turbidimetric Immunoassay Kit

β2-Microglobulin (B2M) Turbidimetric Immunoassay Kit

SKU: 51680
  • INTRODUCTION

    β2-Microglobulin(β2-MG), is a small-molecule globulin produced by lymphocytes, platelets, and polymorphonuclear leukocytes, with a molecular weight of 11,800 and a single-chain polypeptide consisting of 99 amino acids. It is the β chain (light chain) portion of the human leukocyte antigen (HLA) on the cell surface. It contains a pair of disulfide bonds in the molecule and contains no sugar; similar to the structure of the immunoglobulin stable region. It is widely found in plasma, urine, cerebrospinal fluid, saliva, and colostrum. Normal human β2-microglobulin synthesis rate and release from the cell membrane are fairly constant. Β2-microglobulin can be freely filtered from the glomerulus, 99.9% is absorbed in the proximal tubules, and in renal tubular epithelial cells Decomposition and destruction; therefore, the emission of β2-microglobulin is very small under normal circumstances.

    β2-MG mainly reflects glomerular filtration dysfunction, urine β2-MG mainly reflects renal tubular reabsorption function impairment. It also can be used as a tumor marker for some people with blood cell cancers (multiple myeloma, ly -mphoma) to give information about their likely prognosis. The health refere- nce of β2-MG is 0.8-2 mg/L in blood and 0.11-0.32 mg/L in urine.

    IMD developed β2-MG PETIA kit uses its unique antibodies and antigens, with accuracy and specificity fully validated using international standard

  • PRINCIPLE OF THE ASSAY

    This assay is a turbidimetric immunoassay for the quantitative measurement of β2-MG in human serum and plasma. A standard or sample is added into a cuvette and mixed with the reaction buffer R1. After a short incubation, the test reagent R2, which is a suspension of microparticles coated with β2-MG antibodies, is added into the cuvette and mixed. The presence of β2-MG in the standard or sample causes the immune-particles to aggregate. The extent to which the microparticles aggregate is quantified by the amount of light scattering measured as absorbance by a chemistry analyzer. The concentration of β2-MG in unknown samples can be interpolated from a reference curve using the standards provided.

  • PERFORMANCE OF THE KIT

    TYPICAL STANDARD CURVE

    β2-MG  (mg/L)

    Absorbance (570 nm)

    0

    -0.0042

    0.2

    0.0055

    1

    0.0640

    2

    0.1217

    10

    0.4353

    20

    0.6265

  • ASSAY PROCEDURE

    Assay procedures may vary depending on the automated chemistry analyzer to be used. A general example of assay procedures is stated as follow:

    1. Dispense 180µl of R1 into a clean cuvette

    2. Add 2µl of sample and incubate at 37˚C for 5 minutes

    3. Further add 60µl of R2

    4. Read change of absorbance at Main Wavelength 570 nm for 8 minutes after the addition of R2

    5. Calculate the concentration of β2-Microglobulin in unknown sample by interpolation from a reference curve using the standards provided

  • PACKING SPECIFICATIONS

    Cat. No.

    Size

    Approximately tests

    51680-05

    R1: 15ml, R2: 5ml

    100

    51680-10

    R1: 30ml, R2: 10ml

    200

    51680-20

    R1: 60ml, R2: 20ml

    400

    51680-50

    R1: 150ml, R2: 50ml

    1000

    51680-100

    R1: 300ml, R2: 100ml

    2000

    For bulk order, please enquire

  • OTHER MATERIALS REQUIRED

    Product           

    Catalog           Number

    Format

    Method

    Calibrator                 

    51680-S1

    5 x 1ml

     

    Control

    51680-C1

    2 x 1ml

     

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