Adiponectin (mouse), rabbit PAb
Application : ELISA, WB
Size : 100 ug
INTRODUCTION
Adiponectin, also termed gelatin-binding protein-28 (GBP28), AdipoQ, ACP30(Acrp30), or apM, is a major adipocyte-secreted adipokine which abundantly presentin the circulation as three distinct oligomeric complexes: LMW(67kDa),MMW(167kDa) and HMW(300kDa) adiponectin. Its levels are decreased in insulinresistant, diabetes and cardiovascular disease. Conversely, elevation of circulatingadiponectin concentrations can alleviate various vascular dysfunctions in animalmodels, suggesting this adipokine exerts vasculoprotective effects.
In addition,adiponectin may also be of importance in the development and progression ofseveral malignancies.
DATA SHEET
ISOTYPE/PREPARATION
Rabbit crude IgG was purified by protein-G column
IMMUNOGEN
Recombinant full-length human adiponectin expressed in mammalian cells
SPECIFICITY
The antibody detects three types of circular human adiponectin and monomer (30kDa) adiponectin
FORMULATION
Solution in PBS.
STORAGE
Store at –20°C. For long-term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.
APPLICATION/ USAGE
Western blot - This antibody can be used at 0.1 – 0.2 µg/mL with the appropriate secondary reagents to detect mouse/rat adiponectin.
ELISA - This antibody can be used at 0.5 - 1.0 µg/mL with the appropriate secondary reagents to detect mouse/rat adiponectin.
Immunoprecipitation - See reference [1], [2]
PUBLICATIONS CITING IMD ADIPONECTIN:
1- Hui X, Gu P, Zhang J, et al. Cell Metab. 2015; 22(2): 279-290.
2- Zhang, Xu, et al. Cell Death Differ. 2017; 24(2):225-237.REFERENCES
[1] Xu A, et al. (2005) Testosterone selectively reduces the high molecular weight form of adiponectin by
inhibiting its secretion from adipocytes. J. Biol. Chem. 280, 18073–18080
[2] Xu A, et al. (2008) Selective Elevation of Adiponectin Production by the Natural Compounds Derived
from a Medicinal Herb Alleviates Insulin Resistance and Glucose Intolerance in Obese Mice. Endocrinology.
[Epub ahead of print]
[3] Xu A, et al. (2004) Adiponectin ameliorates dyslipidemia induced by the human immunodeficiency virus
protease inhibitor ritonavir in mice. Endocrinology. 145(2):487-94
[4] Wang Y, et al. (2008) Post-translational modifications of adiponectin: mechanisms and functional
implications. Biochem J. 409(3):623-33