Anti-mouse IL-33 Antibody, Rabbit pAb

IL-33 was identified based on sequence and structural homology with IL-1 family cytokines. The N-terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL-1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses. A ternary signaling complex is formed by the subsequent association of IL-33 and ST2L with IL-1R AcP. Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion. In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues.

Recombinant mouse IL-33 expressed in E.coli. (Cat. No.: 41750)

The antibody detects mouse IL-33.

Rabbit specific IgG was purified by antigen affinity chromatography

Store at –20°C. For long-term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.

Affinity chromatography on a column with immobilized antigen.

 Not yet tested in other species

Solution in PBS.

ELISA - This antibody can be used as capture antibody in Mouse IL-33 Elisa (Cat. No.: 31750).