Appl1 (human), rabbit PAb

SKU: 11130

Application  :  WB, IP, IH

Size              :  0.1 mg

  • INTRODUCTION

    APPL1, an adaptor protein containing an NH2-terminal Bin/Amphiphiphysin/Rvs (BAR) domain, a central pleckstrin homology (PH) domain and a COOH-terminal phosphotyrosine binding (PTB) domain [1], was originally identified as an interacting partner of Akt in a yeast two-hybrid assay using Akt2 as a bait [2]. APPL1 binds to a number of cell surface receptors (TrkA[3, 4], DCC[5], adiponectin [6, 7], FSH[8]) and intracellular signaling molecules (small GTPase Rab5[9], GIPC[4] and inositol 5-phosphatase[10], suggesting that APPL1 may act as a common relay to coordinate diverse signaling cascades. APPL1 potentiates insulin-mediated Akt activation by counteracting the effect of the Akt inhibitor TRB3 [11].

     

  • DATA SHEET

  • IMMUNOGEN

    Recombinant full-length human APPL1 expressed in E. coli

  • SPECIFICITY

    The antibody detects several types of APPL1 in different species such as human, monkey, mouse, rat etc. (about 85kDa)

  • FORMULATION

    Solution in PBS.

  • STORAGE

    Store at –20°C. For long-term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.

  • APPLICATION/ USAGE

    Western blot - This antibody can be used at 0.1 - 0.2 µg/mL with the appropriate secondary reagents to detect APPL1.

    Immunostaining - This antibody can be used at 1.0 -2.0 µg/mL with the appropriate secondary reagents to detect APPL1.

    ELISA - This antibody can be used at 2.0 - 5.0 µg/mL with the appropriate secondary reagents to detect APPL1.

    Immunoprecipitation – See reference [6], [11]

  • PUBLICATIONS CITING IMD ADIPONECTIN:

     

     Cheng, Kenneth KY, et al. The adaptor protein APPL2 inhibits insulin-stimulated glucose uptake by interacting with TBC1D1 in skeletal muscle. Diabetes. 2014; 63(11):3748-58.
     Yau, Suk Yu, et al. Physical exercise-induced hippocampal neurogenesis and antidepressant effects are mediated by the adipocyte hormone adiponectin. Proc Natl Acad Sci U S A. 2014; 111(44):15810-5.
     Dadson K, et al. Cellular, structural and functional cardiac remodelling following pressure overload and unloading. Int J Cardiol. 2016; 216:32-42.

     

  • ISOTYPE/PREPARATION

    Rabbit specific IgG was purified by affinity APPL1 coupled column

  • REFERENCE

    1. Hosch, S.E., J.M. Olefsky, and J.J. Kim, APPLied mechanics: uncovering how adiponectin modulates insulin action. Cell Metab, 2006. 4(1): p. 5-6.

    2. Mitsuuchi, Y., et al., Identification of a chromosome 3p14.3-21.1 gene, APPL, encoding an adaptor molecule that interacts with the oncoprotein-serine/threonine kinase AKT2. Oncogene., 1999. 18(35): p. 4891-8.

    3. Lin, D.C., et al., APPL1 associates with TrkA and GIPC1, and is required for NGFmediated signal transduction. Mol Cell Biol, 2006. 25: p. 25.

    4. Varsano, T., et al., GIPC is recruited by APPL to peripheral TrkA endosomes and regulates TrkA trafficking and signaling. Mol Cell Biol, 2006. 26(23): p. 8942-52.

    5. Liu, J., et al., Mediation of the DCC apoptotic signal by DIP13 alpha. J Biol Chem., 2002. 277(29): p. 26281-5. Epub 2002 May 14.

    6. Cheng, K.K., et al., Adiponectin-induced endothelial nitric oxide synthase activation and nitric oxide production are mediated by APPL1 in endothelial cells. Diabetes, 2007. 56(5): p. 1387-94.

    7. Mao, X., et al., APPL1 binds to adiponectin receptors and mediates adiponectin signalling and function. Nat Cell Biol., 2006.

    8(5): p. 516-23. Epub 2006 Apr 16. 8. Nechamen, C.A., et al., Human follicle-stimulating hormone (FSH) receptor interacts with the adaptor protein APPL1 in HEK 293 cells: potential involvement of the PI3K pathway in FSH signaling. Biol Reprod., 2004. 71(2): p. 629-36. Epub 2004 Apr 7.

    9. Miaczynska, M., et al., APPL proteins link Rab5 to nuclear signal transduction via an endosomal compartment. Cell., 2004. 116(3): p. 445-56.

    10. Erdmann, K.S., et al., A role of the Lowe syndrome protein OCRL in early steps of the endocytic pathway. Dev Cell, 2007. 13(3): p. 377-90.

    11. Cheng, K.K., et al., APPL1 potentiates insulin-mediated inhibition of hepatic glucose production and alleviates diabetes via Akt activation in mice. Cell Metab, 2009. 9(5): p. 417-27.

$265.00Price

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