Appl2 (human), rabbit PAb

APPL2, also termed as Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2, has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, APPL1, adiponectin receptors, and proteins of the NuRD/MeCP1 complex.

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Recombinant full-length human APPL2 expressed in E. coli cells

The antibody detects many types of APPL2 in different species such as human, monkey, mouse, rat etc. (about 75kDa)

Solution in PBS with 0.02% sodium azide.

Store at –20°C. For long-term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.

Western blot - This antibody can be used at 0.2 - 0.3 µg/mL with the appropriate secondary reagents to detect APPL2.

Immunostaining - This antibody can be used at 2-3 µg/mL with the appropriate secondary reagents to detect APPL2.

ELISA - This antibody can be used at 1.0 - 2.0 µg/mL with the appropriate secondary reagents to detect APPL2

 Cheng, Kenneth KY, et al. The adaptor protein APPL2 inhibits insulin-stimulated glucose uptake by interacting with TBC1D1 in skeletal muscle. Diabetes. 2014; 63(11):3748-58.
 Yau, Suk Yu, et al. Physical exercise-induced hippocampal neurogenesis and antidepressant effects are mediated by the adipocyte hormone adiponectin. Proc Natl Acad Sci U S A. 2014; 111(44):15810-5.
 Dadson K, et al. Cellular, structural and functional cardiac remodelling following pressure overload and unloading. Int J Cardiol. 2016; 216:32-42.

Rabbit specific IgG was purified by affinity APPL2 coupled column