Anti-human FGF-21 Antibody, Rabbit pAb

Fibroblast growth factor 21 (FGF-21) is a novel protein that has been implicated in the regulation of lipid and glucose metabolism under fasting and ketotic conditions [1,2]. In murine models, FGF-21 is predominantly expressed in liver, but also expressed in adipose tissue and pancreatic β-cells [3,4]. FGF-21 stimulates glucose uptake in adipocytes. It also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents [5]. When administered daily for 6 weeks to diabetic rhesus monkeys, FGF-21 caused a dramatic decline in fasting plasma glucose, fructosamine, triglycerides, insulin and glucagon [6]. Furthermore, elevated plasma FGF-21 concentrations in humans appear to be related to the presence of hepatic and peripheral insulin resistance [7].

Recombinant full-length human FGF-21 expressed in E.coli.

The antibody detects circular human FGF-21.

Liquid in phosphate-buffered saline (PBS).

Store at –20°C for less than one week. For long-term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.

ELISA - The antibody can be used at 2 μg /mL with the appropriate secondary reagents to detect human FGF21.

Antigen affinity-purified

[1] Kharitonenkov A, Shiyanova TL, et al. (2005) J Clin Invest; 115: 1627– 1635

[2] Badman MK, Pissios P, et al. (2007) Cell Metab; 5: 426– 437

[3] Nishimura T, Nakatake Y, et al. (2000) Biochim Biophys Acta; 1492: 203– 206

[4] Kurosu H, Choi M, et al.  (2007) J Biol Chem; 282: 26687– 26695

[5] Kharitonenkov A, Shiyanova TL, et al. (2005) J. Clin. Invest. 115: 1627–35.

[6] Kharitonenkov A, Wroblewski VJ, et al. (2007) Endocrinology;148:774-81

[7] Chavez AO, Molina-Carrion M, et al. (2009) Diabetes Care; 32:1542-6.

1. Ye D, Li H, Wang Y, Jia W, Zhou J, Fan J, Man K, Lo C, Wong C, Wang Y, Lam KS. Circulating fibroblast growth factor 21 is a sensitive biomarker for severe ischemia/reperfusion injury in patients with liver transplantation. Scientific reports. 2016 Jan 25;6(1):1-9.