R179Q (9Human Fibroblast Growth Factor 23)
FGF-23 is a bone-derived hormone that acts in the kidney to regulate phosphate homeostasis and vitamin D metabolism. The signaling receptor for FGF-23, a Klotho-FGFR1 (IIIc) complex, is an essential regulator of the renal sodium phosphate co-transporter and key vitamin D-metabolizing enzymes CYP27B1 and CYP24A1. Mature human FGF23 contains an atypical (very low affinity) heparin binding site (aa 134162), a proteolytic cleavage site (Arg179Ser180), and multiple Olinked glycosylation sites with Thr178 being of particular importance. Olinked glycosylation at Thr178 blocks the cleavage of FGF23, thereby preventing loss of FGF23 activity. This recombinant human FGF23 bears mutation at 179th aa from arginine to glutamine preventing proteolytic cleavage.
Expressed in E.coli with total 271 AA. Mw: 30.4 KDa (calculated).
N-terminal 6xHis-tag, EK and TEV cleavage site, 44 extra AA (highlighted).
Recombinant antigen for research use or manufacturing only.
AMINO ACID SEQUENCE
Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C. Avoid repeated freezing/thawing cycles.
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Lyophilized at 1 mg/mL in 50mM Tris, 300mM NaCl, 400mM arginine.
Standard ELISA test, Western Blot.