Human C-peptide immunoassay kit

Human C-peptide immunoassay kit

SKU: 31780

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Assay range :   3 - 120 uU/ml

Size:                  96T/Kit

 

 

  • INTRODUCTION

    C-peptide is a 31 amino acid peptide which is a part of proinsulin and is cleaved out proteolytically during the formation of insulin. The half-life of C-peptide is longer (20-30 min) than that of insulin (3-5 min), so C-peptide is a more stable parameter while assessing b-cell function1.

  • PRINCIPLE OF THE ASSAY

    This assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a monoclonal antibody specific for human C-peptide. Standards and samples are pipetted into the wells and any human C-peptide present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled monoclonal antibody specific for human C-peptide is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human C-peptide bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human C-peptide, the unknown sample concentration can be interpolated from a reference curve included in each assay.

  • ASSAY PERFORMANCE

    A. Typical representation of standard curve

    The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay. 

     

    Human C-peptide(pg/ml)

    Absorbance

    (450 nm)

    Blanked Absorbance

    0

    0.063

    0

    15.6

    0.117

    0.054

    31.2

    0.182

    0.119

    62.5

    0.298

    0.235

    125

    0.52

    0.457

    250

    0.934

    0.871

    500

    1.576

    1.513

    1000

    2.533

    2.47

     

    B. Sensitivity:

    The lowest level of C-peptide hat can be measured by this assay is 15.6  pg/ml.

     

    C. Precision:

    Intra-assay Precision (Precision within an assay) C.V< 10%.

    Inter-assay Precision (Precision between assays) C.V< 10%.

     

     

     

$330.00Price

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