Human GDF-15 immunoassay kit
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Assay range : 7.8 - 500 pg/mL
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INTRODUCTION
Growth differentiation factor 15 (GDF-15) belongs to transforming growth factor β family1. The biologically active form of GDF-15 is a 28 kDa disulphide linked homodimer2.It is also known as macrophage inhibiting cytokine 1 (MIC-1), placental transformation growth factor (PTGF-b), prostate derived factor (PDF),placental bone morphogenetic protein (PLAB), NSAID activated gene-1 (NAG-1) and PL741.
PRINCIPLE OF THE ASSAY
This assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a monoclonal antibody specific for human GDF-15. Standards and samples are pipetted into the wells and any human GDF-15 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human GDF-15 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human GDF-15 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human GDF-15, the unknown sample concentration can be interpolated from a reference curve included in each assay.
ASSAY PERFORMANCE
A. Typical representation of standard curve
The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.
Human GDF-15 (pg/ml)
Absorbance
(450 nm)
Blanked Absorbance
0
0.074
0
7.8
0.13
0.056
15.6
0.197
0.123
31.2
0.338
0.264
62.5
0.577
0.503
125
1.017
0.943
250
1.719
1.645
500
2.626
2.552
B. Sensitivity:
The lowest level of human GDF-15 that can be detected by this assay is 7.8 pg/ml.
C. Precision:
Intra-assay Precision (Precision within an assay) <10%.
Inter-assay Precision (Precision between assays) <10%.