Human GDF-15 ELISA Kit

Human GDF-15 ELISA Kit

SKU: 31980

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Assay range: 7.8 - 500 pg/mL

Kit Size: 96 wells/kit

Other Names: MIC-1, PTGF-b, PDF, PLAB, NAG-1, PL741

  • INTRODUCTION

    Growth differentiation factor 15 (GDF-15) belongs to transforming growth factor β family1. The biologically active form of GDF-15 is a 28 kDa disulphide linked homodimer2.It is also known as macrophage inhibiting cytokine 1 (MIC-1), placental transformation growth factor (PTGF-b), prostate derived factor (PDF),placental bone morphogenetic protein (PLAB), NSAID activated gene-1 (NAG-1) and PL741.

  • PRINCIPLE OF THE ASSAY

    This assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a monoclonal antibody specific for human GDF-15. Standards and samples are pipetted into the wells and any human GDF-15 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human GDF-15 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human GDF-15 bound initially.  The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human GDF-15, the unknown sample concentration can be interpolated from a reference curve included in each assay.

  • ASSAY PERFORMANCE

    A. Typical representation of standard curve

    The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay. 

    Human GDF-15 (pg/mL)

    Absorbance

    (450 nm)

    Blanked Absorbance

    0

    0.074

    0

    7.8

    0.13

    0.056

    15.6

    0.197

    0.123

    31.2

    0.338

    0.264

    62.5

    0.577

    0.503

    125

    1.017

    0.943

    250

    1.719

    1.645

    500

    2.626

    2.552

     

    B. Sensitivity

    The lowest level of human GDF-15 that can be detected by this assay is 7.8 pg/mL.

     

    C. Precision

    Intra-assay Precision (Precision within an assay) C.V. <10%.

    Inter-assay Precision (Precision between assays) C.V. <10%.

  • PUBLICATIONS CITING THIS PRODUCT

    1. Wu X, Xuan W, You L, Lian H, Li F, Zhang X, Chen Q, Sun K, Chen C, Xu M, Li Y, Yan L, Zhang X, Ren M. Associations of GDF-15 and GDF-15/adiponectin ratio with odds of type 2 diabetes in the Chinese population. Endocrine. 2021 Mar 12. doi: 10.1007/s12020-021-02632-1. Epub ahead of print. PMID: 33713014.
$390.00Price

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