Human GDF-15 immunoassay kit

Human GDF-15 immunoassay kit

SKU: 31980

 

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Assay range : 7.8 - 500 pg/mL

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  • INTRODUCTION

    Growth differentiation factor 15 (GDF-15) belongs to transforming growth factor β family1. The biologically active form of GDF-15 is a 28 kDa disulphide linked homodimer2.It is also known as macrophage inhibiting cytokine 1 (MIC-1), placental transformation growth factor (PTGF-b), prostate derived factor (PDF),placental bone morphogenetic protein (PLAB), NSAID activated gene-1 (NAG-1) and PL741.

  • PRINCIPLE OF THE ASSAY

    This assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a monoclonal antibody specific for human GDF-15. Standards and samples are pipetted into the wells and any human GDF-15 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human GDF-15 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human GDF-15 bound initially.  The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human GDF-15, the unknown sample concentration can be interpolated from a reference curve included in each assay.

  • ASSAY PERFORMANCE

    A. Typical representation of standard curve

    The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay. 

    Human GDF-15 (pg/ml)

    Absorbance

    (450 nm)

    Blanked Absorbance

    0

    0.074

    0

    7.8

    0.13

    0.056

    15.6

    0.197

    0.123

    31.2

    0.338

    0.264

    62.5

    0.577

    0.503

    125

    1.017

    0.943

    250

    1.719

    1.645

    500

    2.626

    2.552

     

    B. Sensitivity:

     The lowest level of human GDF-15 that can be detected by this assay is 7.8 pg/ml.

     

    C. Precision:

    Intra-assay Precision (Precision within an assay) <10%.

    Inter-assay Precision (Precision between assays) <10%.

$390.00Price

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