Human PAI-1 immunoassay kit
Human plasminogen activator inhibitor type-1 ELISA kit
Assay range : 0.016-2 ng/ml
Size : 96T/Kit
The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.
INTRODUCTION
Plasminogen activator inhibitor-1 (PAI-1) is the primary inhibitor of tissue-type and urokinase-type plasminogen activator, playing a major role in fibrinolysis1-2 . PAI-1 is mainly produced by the endothelium, but is also secreted by other tissue types, such as adipose tissue3 . It is normally present at low levels in plasma and tissue, but its expression and release increased in various disease states (such as a number of forms of cancer), as well as in obesity and the metabolic syndrome4 . PAI-1 is also involved in the pathophysiology of renal, pulmonary, cardiovascular, and metabolic diseases 5-8 . Elevated local or systemic PAI-1 can also exacerbate such pathologic conditions.
ASSAY CHARACTERISTICS
A. Sensitivity:
The lowest level of PAI-1 that can be measured by this assay is 0.031 ng/ml.
B. Specificity:
The antibodies used in this assay are specific to human PAI-1 and do not cross-react with mouse and rat PAI-1, and other cytokine or hormone molecules including human resistin, TNFα, ANGPTL4, insulin, leptin and IL6.
C. Precision:
Intra-assay Precision (Precision within an assay)
Two samples of known concentration were tested 16 times on one plate
Inter-assay Precision (Precision between assays)
Four samples of known concentration were tested in 8 separate assays.
D. Recovery:
Serum samples were spiked with different amounts of human PAI-1 and assayed.
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DATA SHEET