Human PAI-1 ELISA Kit
Human plasminogen activator inhibitor type-1 ELISA kit
Assay range : 0.016-2 ng/ml
Kit Size : 96T/Kit
Other Names: SERPINE1, PLANH-1, PAI
The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.
Plasminogen activator inhibitor-1 (PAI-1) is the primary inhibitor of tissue-type and urokinase-type plasminogen activator, playing a major role in fibrinolysis . PAI-1 is mainly produced by the endothelium, but is also secreted by other tissue types, such as adipose tissue. It is normally present at low levels in plasma and tissue, but its expression and release increased in various disease states (such as a number of forms of cancer), as well as in obesity and the metabolic syndrome. PAI-1 is also involved in the pathophysiology of renal, pulmonary, cardiovascular, and metabolic diseases. Elevated local or systemic PAI-1 can also exacerbate such pathologic conditions.
The lowest level of PAI-1 that can be measured by this assay is 0.031 ng/ml.
The antibodies used in this assay are specific to human PAI-1 and do not cross-react with mouse and rat PAI-1, and other cytokine or hormone molecules including human resistin, TNFα, ANGPTL4, insulin, leptin and IL6.
Intra-assay Precision (Precision within an assay)
Two samples of known concentration were tested 16 times on one plate
Inter-assay Precision (Precision between assays)
Four samples of known concentration were tested in 8 separate assays.
Serum samples were spiked with different amounts of human PAI-1 and assayed.