Human TIMP-1 Immunoassay kit

Human TIMP-1 Immunoassay kit

SKU: 31103

 

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Assay range :  31.2 - 2000 pg/ml

Size:                  96T/Kit

 

  • INTRODUCTION

     

    Tissue inhibitor of metalloproteinases-1(TIMP-1) plays a crucial role in extracellular matrix (ECM) composition and wound healing. It has been found to be strongly upregulated in liver tissue and serum during hepatic fibrogenesis in patients with liver disease and in animal models of hepatic fibrogenesis.

  • PRINCIPLE OF THE ASSAY

    This assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a monoclonal antibody specific for human TIMP-1. Standards and samples are pipetted into the wells and any human TIMP-1 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human TIMP-1 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human TSP-2 bound initially.  The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human TIMP-1, the unknown sample concentration can be interpolated from a reference curve included in each assay.

  • ASSAY PERFORMANCE

    A. Typical representation of standard curve

    The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.  

    Human TIMP-1 (pg/ml)

    Absorbance 

    (450 nm)

    Blanked Absorbance

    0

    0.091

    0

    31.2

    0.118

    0.027

    62.5

    0.161

    0.07

    125

    0.257

    0.166

    250

    0.449

    0.358

    500

    0.876

    0.785

    1000

    1.544

    1.453

    2000

    2.494

    2.403


     

    B. Sensitivity:

    The lowest level of human adiponectin that can be detected by this assay is 15.6 pg/ml.

     

    C. Precision:

    Intra-assay Precision (Precision within an assay) <10%.
    Inter-assay Precision (Precision between assays) <10%.

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