Humanized Monoclonal Antibody (IgG) against SARS-CoV-2 S1RBD
Cat. No.: 41A244 Size: 100µg
Type: Genetically engineered recombinant monoclonal IgG antibody
The SARS-CoV-2 glycosylated spike (S) protein highly exposed on the viral surface is a major determinant for virus binding and invasion into host cells, which is a main target for neutralization antibody. The receptor-binding domain (RBD) in SARS-CoV-2 S protein is responsible for binding to human and bat angiotensin-converting enzyme 2 (ACE2) receptors.
The cDNA encoding the highly variable region of the antibody recognizing S1RBD from mammalian cells were identified by phage display library, fused with the Fc fragment of human IgG1, and expressed in Chinese Hamster Ovary (CHO) cells.
FORMULATION, RECONSTITUTION AND STORAGE
Lyophilized from sterile PBS, pH 7.4. Lyophilized protein can be stored at 2℃ to 8℃ for short-term, and at -20℃ to -80℃ for long term. For reconstitution, add 100 μl of deionized water, mix gently and incubate the reconstituted product for 10 minutes at room temperature prior to use. Reconstituted protein should be kept at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.
ELISA and other applications. Optimal concentration/dilution should be determined by the end user.
PUBLICATIONS CITING THIS PRODUCT
- Zhu Z, Ma X, Zhu L, Luo Q, Lin N, Chen Z, Zhao X, Lin Z, Cai Z. Equipment-free, gold nanoparticle based semiquantitative assay of SARS-CoV-2-S1RBD IgG from fingertip blood: A practical strategy for on-site measurement of COVID-19 antibodies. Talanta. 2022 Aug 15;246:123498.