Mouse Adiponectin ELISA Kit

Mouse Adiponectin ELISA Kit

SKU: 32010

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Assay range: 3.12 - 200 ng/mL

Kit Size: 96 wells/kit

Other Names: Acrp30, AdipoQ, apM1, GBP-28

  • INTRODUCTION

    Adiponectin is an adipokine exclusively expressed in adipose tissues with potent anti-diabetic, anti-atherogenic and anti-inflammatory functions.
    In humans, decreased serum adiponectin levels are associated with increased body mass index (BMI), decreased insulin sensitivity, less favourable plasma lipid profiles, increased inflammation and increased risk for the development of type 2 diabetes, hypertension and coronary heart diseases

  • PRINCIPLE OF THE ASSAY

    This assay is a sandwich ELISA using affinity-purified polyclonal antibodies against mouse adiponectin. The immunoplate is pre-coated with anti-mouse adiponectin capture antibody. Standards and samples are pipetted into the wells and any mouse adiponectin present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase (HRP)-linked polyclonal antibody specific for mouse adiponectin is added to the wells. After a final wash step, an HRP substrate solution is added and colour develops in proportion to the amount of mouse adiponectin bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured mouse adiponectin, the unknown sample concentration can be interpolated from a reference curve included in each assay.

  • ASSAY PERFORMANCE

    A. Typical representation of standard curve

    The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.

    Adiponectin (ng/mL)

    Absorbance (450 nm)

    Blanked Absorbance

    0

    0.064

    0

    3.12

    0.105

    0.041

    6.25

    0.151

    0.087

    12.5

    0.257

    0.193

    25

    0.467

    0.403

    50

    0.801

    0.737

    100

    1.463

    1.399

    200

    2.393

    2.329

     

    B. Sensitivity

    The lowest level of mouse adiponectin that can be detected by this assay is 3.12 ng/mL.

     

    C. Specificity

    The antibodies used in this assay are specific to mouse adiponectin and do not cross-react with human adiponectin, and other cytokine or hormone molecules.

  • PUBLICATIONS CITING THIS PRODUCT

    1. Yau SY, Li A, Hoo RL, Ching YP, Christie BR, Lee TM, Xu A, So KF. Physical exercise-induced hippocampal neurogenesis and antidepressant effects are mediated by the adipocyte hormone adiponectin. Proceedings of the National Academy of Sciences. 2014 Nov 4;111(44):15810-5.
    2. Lin Z, Pan X, Wu F, Ye D, Zhang Y, Wang Y, Jin L, Lian Q, Huang Y, Ding H, Triggle C. Fibroblast growth factor 21 prevents atherosclerosis by suppression of hepatic sterol regulatory element-binding protein-2 and induction of adiponectin in mice. Circulation. 2015 May 26;131(21):1861-71.
    3. Hui X, Gu P, Zhang J, Nie T, Pan Y, Wu D, Feng T, Zhong C, Wang Y, Lam KS, Xu A. Adiponectin enhances cold-induced browning of subcutaneous adipose tissue via promoting M2 macrophage proliferation. Cell metabolism. 2015 Aug 4;22(2):279-90.
    4. Shu L, Hoo RL, Wu X, Pan Y, Lee IP, Cheong LY, Bornstein SR, Rong X, Guo J, Xu A. A-FABP mediates adaptive thermogenesis by promoting intracellular activation of thyroid hormones in brown adipocytes. Nature communications. 2017 Jan 27;8(1):1-6.
    5. Li H, Wu G, Fang Q, Zhang M, Hui X, Sheng B, Wu L, Bao Y, Li P, Xu A, Jia W. Fibroblast growth factor 21 increases insulin sensitivity through specific expansion of subcutaneous fat. Nature communications. 2018 Jan 18;9(1):1-6.
    6. Zhang X, Cheng Q, Wang Y, Leung PS, Mak KK. Hedgehog signaling in bone regulates whole-body energy metabolism through a bone–adipose endocrine relay mediated by PTHrP and adiponectin. Cell Death & Differentiation. 2017 Feb;24(2):225-37.
    7. Pan Y, Hui X, Hoo RL, Ye D, Chan CY, Feng T, Wang Y, Lam KS, Xu A. Adipocyte-secreted exosomal microRNA-34a inhibits M2 macrophage polarization to promote obesity-induced adipose inflammation. The Journal of clinical investigation. 2019 Feb 1;129(2):834-49.
    8. Geng L, Liao B, Jin L, Huang Z, Triggle CR, Ding H, Zhang J, Huang Y, Lin Z, Xu A. Exercise alleviates obesity-induced metabolic dysfunction via enhancing FGF21 sensitivity in adipose tissues. Cell reports. 2019 Mar 5;26(10):2738-52.
    9. Ge MQ, Yeung SC, Mak JC, Ip MS. Differential metabolic and inflammatory responses to intermittent hypoxia in substrains of lean and obese C57BL/6 mice. Life sciences. 2019 Dec 1;238:116959.
    10. Wang P, Liang Y, Chen K, Yau SY, Sun X, Cheng KK, Xu A, So KF, Li A. Potential involvement of adiponectin signaling in regulating physical exercise-elicited hippocampal neurogenesis and dendritic morphology in stressed mice. Frontiers in cellular neuroscience. 2020 Jul 3;14:189.
    11. Lee TH, Christie BR, van Praag H, Lin K, Siu PM, Xu A, So KF, Yau SY. AdipoRon Treatment Induces a Dose-Dependent Response in Adult Hippocampal Neurogenesis. International Journal of Molecular Sciences. 2021 Jan;22(4):2068.
    12. Zhang Y, Cao H, Chen J, Li Y, Xu A, Wang Y. Adiponectin-expressing Treg facilitate T lymphocyte development in thymic nurse cell complexes. Communications biology. 2021 Mar 16;4(1):1-7.
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