MUP-1 (Mouse Major Urinary Protein 1) ELISA kit
Major urinary protein 1 (Mup-1), also known as Mup7, Up-1, Ltn-1, Mup-1, Mup-a, Mup10 and Lvtn-1, is a low molecular weight secreted protein produced predominantly from the liver. Structurally it belongs to the lipocalin family, which carries small hydrophobic ligands such as pheromones. It has been demonstrated that Mup-1 is an important player in regulating energy expenditure and metabolism in mice. In both dietary and genetic obese mice, the circulating concentrations of Mup1 were markedly decreased compared with the lean controls. Replenishment of recombinant MUP-1 led to improved glucose tolerance and insulin sensitivity, as well as increased energy expenditure and locomotor activity in db/db diabetic mice. It was suggested that Mup1 also regulates systemic glucose and/or lipid metabolism through the paracrine/autocrine regulation of the hepatic gluconeogenic and/or lipogenic programs, respectively.
PRINCIPLE OF THE ASSAY
This assay is a sandwich ELISA designed for the quantitative detection of mouse Mup1 in samples. The immunoplate is pre-coated with antibody specific to mouse Mup1. Standards and samples are pipetted into the wells and any mouse Mup1 present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase (HRP)-linked polyclonal antibody specific for mouse Mup1 is added to the wells. After a final wash step, an HRP substrate solution is added and colour develops in proportion to the amount of mouse Mup1 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured mouse Mup1, the unknown sample concentration can be interpolated from a reference curve included in each assay.
A. Typical representation of standard curve
The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.
Absorbance (450 nm)
The lowest level of mouse Mup1 that can be detected by this assay is 0.625 ng/ml.
The antibodies used in this assay are specific to mouse Mup1.