Rapid Human Cystatin C ELISA kit
Assay range : 0.78 -100 ng/ml
Size : 96T/Kit
The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.
C-reactive protein (CRP) is a circulating protein mainly secreted from the liver. This acute phase protein consists of five identical non-glycosylated subunits of 23 kDa, that give rise to a symmetrically arranged globular protein with molecular weight of approximately 120 kDa.1 It has long been recognized that CRP is closely related to immunology, inflammation and host defence; as a result it has been used as an inflammatory marker. However, the development of high-sensitivity CRP (hsCRP) ELISA had addressed its role in other clinical issues. There is accumulating evidence suggesting the important role that CRP plays in mediating cardiovascular diseases (CVD) and type 2 diabetes.2-4 Normally CRP is presenting only in a trace amount in circulation (<1 µg/ml)5,6 but can increase over 1,000-fold under acute inflammatory state. Individual with blood CRP levels <1 µg/ml, 1-3 µg/ml and >3 µg/ml is considered to have low, moderate and high risk, respectively, of CVD and myocardial infraction.7 Therefore, blood CRP level has become a promising measure of CVD risk.8,9
The lowest level of human CRP that can be detected by this assay is 0.23 ng/ml.
The antibody pair used in this assay is specific to human CRP and does not cross-react with mouse or rat CRP, or other cytokine and hormone molecules including human resistin, TNFα, ANGPTL4, insulin, leptin and IL6.
Intra-assay Precision (Precision within an assay)
Two samples of known concentration were tested 16 times on one plate.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in 10 separate assays.
Serum samples were spiked with different amounts of human CRP and assaye
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