SARS-CoV-2 Spike S1 Protein (expressed in HEK293 cells)
Tag: Fc tag at C-terminus
Cat. No.: 41A230
Size: 0.1 mg
Endotoxin: <5 EU/mg, determined by the LAL method
INTRODUCTION TO THE MOLECULE
The spike (S) glycoprotein of SARS-CoV-2, which is highly exposed on the viral surface, plays a major role in inducing neutralizing antibody, T cell responses and protective immunity. The S protein contains two subunits: S1 and S2 cleaved by furin in host cells. S1 mediates the interaction of SARS-CoV-2 to the host cell receptor (ACE2) through its receptor-binding domain and is the target of vaccine development.
The recombinant SARS-CoV-2 Spike S1 Protein (YP_009724390.1) (Val16-Arg685) with C-terminal Fc-tag consists of 681 amino acids. Its predicted molecular mass of 77 kDa. The apparent molecular mass of S1 in SDS-PAGE is around 130 kDa, probably due to glycosylation. The concentration of protein was determined by BCA.
BIOACTIVITY AND ANTIGENICITY
Strong binding ability with human ACE2 protein and binding capacity to a human anti-S1 monoclonal antibody (determined by ELISA).
FORMULATION, RECONSTITUTION AND STORAGE
Lyophilized from sterile PBS, pH 7.4. Lyophilized protein can be stored at 2℃ to 8℃ for short-term, and at -20℃ to -80℃ for long term store. For reconstitution, add 100 μl of deionized water, mix gently and incubate the reconstituted product for 10 minutes at room temperature prior to use. Reconstituted protein should be kept at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.