SARS-CoV-2 Spike S1 protein (expressed in HEK293 cells)
Tag: His at C-terminus
Cat. No.: 41A230
Size: 100 ug
Endotoxin: <5 EU/mg, determined by the LAL method
INTRODUCTION TO THE MOLECULE
The spike (S) glycoprotein of SARS-CoV-2, which is highly exposed on the viral surface, plays a major role in inducing neutralizing antibody, T cell responses and protective immunity. The S protein contains two subunits: S1 and S2 cleaved by furin in host cells. S1 mediates the interaction of SARS-CoV-2 to the host cell receptor (ACE2) through its receptor-binding domain and is the target of vaccine development.
The recombinant SARS-CoV-2 Spike S1 Protein (YP_009724390.1) (Val16-Arg685) with C-terminal His-tag consists of 681 amino acids. Its predicted molecular mass of 77 kDa. The apparent molecular mass of S1 in SDS-PAGE is around 120 kDa, probably due to glycosylation. The concentration of protein was determined by BCA.
BIOACTIVITY AND ANTIGENICITY
Strong binding ability with human ACE2 protein and binding capacity to a human anti-S1 monoclonal antibody (determined by ELISA).
FORMULATION AND RECONSTITUTION
The protein is lyophilized in PBS, PH7.4. Add appropriate volume of PBS into the tube and let the pellet dissolve completely. For example, adding 100 ul of PBS to make a final concentration of 1 mg/ml.
Store at -80°C. Recommend to aliquot the protein into smaller quantities. Avoid repeated freeze-thaw cycles.