Ultra sensitive Mouse Insulin ELISA Kit

Ultra sensitive Mouse Insulin ELISA Kit

SKU: 32380

★ Download Datasheet PDF ★

Download MSDS

 

Assay range: 0.025 - 1.5 ng/mL

Sample Size: 25 uL

Kit Size: 96 wells/kit

  • INTRODUCTION

    Insulin is a peptide hormone exclusively produced in pancreatic beta-cells. It consists of A chain and B chain, which are linked by two disulfide bridges.
    Insulin is the primary hormone responsible for glucose metabolism. Impaired insulin secretion and insulin resistance are key causes of type 2 diabetes (T2D).

  • PRINCIPLE OF THE ASSAY

    This assay is a two-site ELISA. The micro-plate is pre-coated with a monoclonal antibody against insulin. Standards and samples are added into the wells and coincubated with a monoclonal antibody conjugated to horseradish peroxidase (HRP) enzyme. After wash step to remove any unbound substances, TMB substrate is added and colour develops in proportion to the amount of insulin bound initially. The assay is stopped and the optical density of the wells determined using a micro-plate reader. Since the increases in absorbance are directly proportional to the amount of captured insulin, the unknown sample concentration can be interpolated from a reference curve included in each assay.

  • ASSAY PERFORMANCE

    A.Typical representation of standard curve

    The following standard curve is provided for demonstration only. A standard curve should be generated for each assay.

     

    Insulin (ng/mL)

    Absorbance

    (450 nm)

    Blanked Absorbance

    0

    0.061

    0

    0.025

    0.07

    0.009

    0.075

    0.096

    0.035

    0.2

    0.223

    0.162

    0.5

    0.77

    0.709

    1.5

    2.852

    2.791

     

    B.Sensitivity

    The lowest insulin level that can be measured by this assay is 0.025 ng/mL.

     

    C. Precision

    Intra-assay Precision (Precision within an assay) C.V. < 2.9%.

    Inter-assay Precision (Precision between assays) C.V. <4.9%.

     

    D. Recovery

    The recovery of the assay was determined by adding various amounts insulin to a sample. The measured concentration of the spiked sample in the assay was compared to the expected concentration. The average recovery was 92%.

     

    E. Specificity

    Percent of cross reactivity

    Human insulin: 100%

    Rat insulin: 100%

  • PUBLICATIONS CITING THIS PRODUCT

    1. Shu L, Hoo RL, Wu X, Pan Y, Lee IP, Cheong LY, Bornstein SR, Rong X, Guo J, Xu A. A-FABP mediates adaptive thermogenesis by promoting intracellular activation of thyroid hormones in brown adipocytes. Nature communications. 2017 Jan 27;8(1):1-6.
    2. Wang B, Lin H, Li X, Lu W, Kim JB, Xu A, Cheng KK. The adaptor protein APPL2 controls glucose-stimulated insulin secretion via F-actin remodeling in pancreatic β-cells. Proceedings of the National Academy of Sciences. 2020 Nov 10;117(45):28307-15.
$430.00Price

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