The Wide Range C-Reactive Protein (wr-CRP) Turbidimetric Immunoassay Kit

The Wide Range C-Reactive Protein (wr-CRP) Turbidimetric Immunoassay Kit

SKU: 51120
  • INTRODUCTION

    C-Reactive Protein (CRP) is an annular pentameric protein of hepatic origin. C-reactive protein is accepted in clinical use as a major, although rather non-specific, marker of inflammation. In generally healthy people, CRP levels are usually less than 5 mg/L. In pathology, CRP concentration has an eno In generally healthy  individuals, the circulating concentration of CRP ranges between 0.8 mg/L to 2.0 mg/L. CRP concentrations between 2 and 10 mg/L are considered as chronic inflammation, between 10-25 mg/L is  suggestive of viral infection, whereas the highest levels of CRP (above 30 mg/L) are observed in bacterial infection, such as septic arthritis, meningitis and pneumonia. CRP level falls rapidly after successful antibiotic treatment, and monitoring CRP levels can be used to monitor the therapeutic outcomes.  Additionally circulating CRP can be used for stratification of cardiovascular risk (see Cat# 51121)

    Full range CRP PETIA kit developed by IMD can accurately measure CRP with an extremely wide range of concentration (0.1 mg-300 mg/L), and therefore can be used for assessment of both chronic inflammation and acute viral/bacterial infection. The assay is validated with international standard.  

  • PRINCIPLE OF THE ASSAY

    This assay is a turbidimetric immunoassay for the quantitative measurement of CRP in human serum and plasma.  A standard or sample is added into a cuvette and mixed with the reaction buffer R1. After a short incubation, the test reagent R2, which is a suspension of microparticles coated with CRP antibodies, is added into the cuvette and mixed. The presence of CRP in the standard or sample causes the immune-particles to aggregate. The extent to which the microparticles aggregate is quantified by the amount of light scattering measured as absorbance by a chemistry analyzer. The concentration of CRP in unknown samples can be interpolated from a reference curve using the standards provided.

  • PERFORMANCE OF THE KIT

    TYPICAL STANDARD CURVE 

    CRP (mg/L)

    Absorbance (570 nm)

    0

    -0.0018

    0.5

    0.0020

    8

    0.0221

    20

    0.0504

    100

    0.2997

    300

    0.5726

  • ASSAY PROCEDURE

    Assay procedures may vary depending on the automated chemistry analyzer to be used. A general example of assay procedures is stated as follow:

    1. Dispense 300µl of R1 into a clean cuvette

    2. Add 2µl of sample and incubate at 37˚C for 5 minutes

    3. Further add 100µl of R2

    4. Read change of absorbance at Main Wavelength 570 nm and Sub Wavelength 800 nm for 8 minutes after the addition of R2

    5. Calculate the concentration of CRP in unknown sample by interpolation from a reference curve using the standards provided

  • PACKING SPECIFICATIONS

    Cat. No.

    Size

    Approximately tests

    51120-05

    R1: 15ml, R2: 5ml

    100

    51120-10

    R1: 30ml, R2: 10ml

    200

    51120-20

    R1: 60ml, R2: 20ml

    400

    51120-50

    R1: 150ml, R2: 50ml

    1000

    51120-100

    R1: 300ml, R2: 100ml

    2000

    For bulk order, please enquire

  • OTHER MATERIALS REQUIRED

    Product           

    Catalog           Number

    Format

    Method   

    Calibrator                 

    51120-S1

    5 x 1ml

     

    Control

    51120-C1

    2 x 1ml

     

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