C-Reactive Protein (CRP) is an annular pentameric protein of hepatic origin. C-reactive protein is accepted in clinical use as a major, although rather non-specific, marker of inflammation. In generally healthy people, CRP levels are usually less than 5 mg/L. In pathology, CRP concentration has an eno In generally healthy  individuals, the circulating concentration of CRP ranges between 0.8 mg/L to 2.0 mg/L. CRP concentrations between 2 and 10 mg/L are considered as chronic inflammation, between 10-25 mg/L is  suggestive of viral infection, whereas the highest levels of CRP (above 30 mg/L) are observed in bacterial infection, such as septic arthritis, meningitis and pneumonia. CRP level falls rapidly after successful antibiotic treatment, and monitoring CRP levels can be used to monitor the therapeutic outcomes.  Additionally circulating CRP can be used for stratification of cardiovascular risk (see Cat# 51121)

Full range CRP PETIA kit developed by IMD can accurately measure CRP with an extremely wide range of concentration (0.1 mg-300 mg/L), and therefore can be used for assessment of both chronic inflammation and acute viral/bacterial infection. The assay is validated with international standard.  

This assay is a turbidimetric immunoassay for the quantitative measurement of CRP in human serum and plasma.  A standard or sample is added into a cuvette and mixed with the reaction buffer R1. After a short incubation, the test reagent R2, which is a suspension of microparticles coated with CRP antibodies, is added into the cuvette and mixed. The presence of CRP in the standard or sample causes the immune-particles to aggregate. The extent to which the microparticles aggregate is quantified by the amount of light scattering measured as absorbance by a chemistry analyzer. The concentration of CRP in unknown samples can be interpolated from a reference curve using the standards provided.

TYPICAL STANDARD CURVE 

CRP (mg/L)	Absorbance (570 nm)
0	-0.0018
0.5	0.0020
8	0.0221
20	0.0504
100	0.2997
300	0.5726

Assay procedures may vary depending on the automated chemistry analyzer to be used. A general example of assay procedures is stated as follow:

1. Dispense 300µl of R1 into a clean cuvette

2. Add 2µl of sample and incubate at 37˚C for 5 minutes

3. Further add 100µl of R2

4. Read change of absorbance at Main Wavelength 570 nm and Sub Wavelength 800 nm for 8 minutes after the addition of R2

5. Calculate the concentration of CRP in unknown sample by interpolation from a reference curve using the standards provided

Cat. No.	Size	Approximately tests
51120-05	R1: 15ml, R2: 5ml	100
51120-10	R1: 30ml, R2: 10ml	200
51120-20	R1: 60ml, R2: 20ml	400
51120-50	R1: 150ml, R2: 50ml	1000
51120-100	R1: 300ml, R2: 100ml	2000

For bulk order, please enquire

Product	Catalog           Number	Format	Method
Calibrator	51120-S1	5 x 1ml
Control	51120-C1	2 x 1ml