Adiponectin (ADPN) Turbidimetric Immunoassay Kit

Adiponectin (ADPN) Turbidimetric Immunoassay Kit

SKU: 51010

 

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★ Download MSDS ★

 

CE Certificate: nqa No. 02891        

CFDA Certificate: No. 20182400947

 

  • INTRODUCTION

    Adiponectin (ADPN), also known as apM1, Acrp30, GBP28 and adipoQ, is a circulating hormone predominantly produced from adipose tissue (fat). Many pharmacological studies demonstrated that this protein possesses potent anti-diabetic, anti-atherogenic and anti-inflammatory functions. Supplementation of adiponectin protein can decrease blood glucose, improve insulin sensitivity, alleviate fatty liver and prevent atherosclerosis1. Decreased circulating levels of plasma adiponectin (hypoadiponectinaemia) are associated with increased body mass index (BMI), and decreased insulin sensitivity. A large number of longitudinal studies in different ethnic groups uniformly demonstrated that circulating adiponectin levels are decreased significantly in type 2 diabetes (T2D) and related complications and low circulating adiponectin levels indicate the increased risk for the development of T2D2. It is generally accepted that the higher the level of adiponectin, the lower the risk of developing diabetes. The level less than 4 mg/L in female or less than 3.5 mg/L in male indicates significant increased risk of developing diabetes.

    Adiponectin PETIA kit developed by IMD takes advantage of its patented proprietary recombinant antigen and antibodies, and can accurately measure adiponectin in serum/plasma using any automatic chemical analyser.

  • PRINCIPLE OF THE ASSAY

    This assay is a turbidimetric immunoassay for the quantitative measurement of adiponectin in human serum and plasma. A standard or sample is added into a cuvette and mixed with the reaction buffer R1. After a short incubation, the test reagent R2, which is a suspension of microparticles coated with an antibody highly specific to adiponectin, is added into the cuvette and mixed. The presence of adiponectin in the standard or sample causes the immune-particles to aggregate. The extent to which the microparticles aggregate is quantified by the amount of light scattering measured as absorbance by a chemistry analyzer. The concentration of adiponectin in unknown samples can be interpolated from a reference curve using the standards provided.

  • PERFORMANCE OF THE KIT

    TYPICAL STANDARD CURVE

    ADPN (mg/L)

    Absorbance (570 nm)

    0

    -0.0099

    2

    0.0264

    5

    0.0882

    10

    0.1872

    20

    0.3359

    40

    0.4906

  • ASSAY PROCEDURE

    Assay procedures may vary depending on the automated chemistry analyzer to be used. A general example of assay procedures is stated as follow:

    1. Dispense 150µl of R1 into a clean cuvette

    2. Add 1.5µl of serum or plasma sample or adiponectin calibrator and incubate at 37˚C for 5 minutes

    3. Further add 50µl of R2

    4. Read change of absorbance at 570 nm for 8 minutes after the addition of R2

    5. Calculate the concentration of Adiponectin in unknown sample by interpolation from a reference curve using the standards provided

  • PACKING SPECIFICATIONS

    Cat. No.

    Size

    Approximately tests

    51010-05

    R1: 15ml, R2: 5ml

    100

    51010-10

    R1: 30ml, R2: 10ml

    200

    51010-20

    R1: 60ml, R2: 20ml

    400

    51010-50

    R1: 150ml, R2: 50ml

    1000

    51010-100

    R1: 300ml, R2: 100ml

    2000

    For bulk order, please enquire

  • OTHER MATERIALS REQUIRED

    Product           

    Catalog           Number

    Format

    Method

    Calibrator                 

    51010-S1

    5 x 1ml

     

    Control

    51010-C1

    2 x 1ml

     

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