Anti-human Angiopoietin-like Protein 4 Antibody, Rabbit pAb
Polyclonal Antibody against Human Angiopoietin-like Protein 4
Application: WB, ELISA
Size: 0.1 mg
Angiopoietin-like protein 4 (ANGPTL4), also known as PPARγ angiopoietin-related protein, fasting-induced adipose factor, or hepatic fibrinogen/ angiopoietin-related protein (HFARP), is an adipokine predominantly expressed in adipose tissue and liver. The experimental results show that ANGPTL4 is a blood-borne hormone directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. Serum levels of ANGPTL4 were decreased in patients with type 2 diabetes. In animal experiments, ANGPTL4 treatments might reduce hyperglycemia, and improve glucose tolerance by decreasing hepatic glucose production and enhancing insulin-mediated inhibition of gluconeogenesis. However, the molecular mechanisms underlying its metabolic actions remain elusive.
Rabbit crude IgG was purified by protein-G chromatography.
Recombinant full-length human ANGPTL4 expressed in E.coli.
The antibody detects human ANGPTL4.
Liquid in phosphate-buffered saline (PBS).
Store at –20°C for less than one week. For long-term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.
Western blot - This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human ANGPTL4.
ELISA - This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human ANGPTL4.
 Xu A, et al. (2005) Testosterone selectively reduces the high molecular weight form of adiponectin by inhibiting its secretion from adipocytes. J. Biol. Chem. 280, 18073–18080
 Wang Y, et al. (2007) Overexpression of angiopoietin-like protein 4 alters mitochondria activities and modulates methionine metabolic cycle in the liver tissues of db/db diabetic mice. Mol Endocrinol. 21(4):972-86.