CRP protein
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INTRODUCTION
C-reactive protein (CRP) is a circulating protein mainly secreted from the liver. This acute phase protein consists of five identical non-glycosylated subunits of 23 kDa, that give rise to a symmetrically arranged globular protein with molecular weight of approximately 120 kDa.1 It has long been recognized that CRP is closely related to immunology, inflammation and host defence, as a result it has been used as an inflammatory marker. There is accumulating evidence suggesting the important role that CRP plays in mediating cardiovascular diseases (CVD) and type 2 diabetes.2-4 Normally CRP is presenting only in a trace amount in circulation (<1 mg/ml)5-6 but can increase over 1,000-fold under acute inflammatory state. Individual with blood CRP levels <1 mg/ml, 1-3 mg/ml and >3 mg/ml is considered to have low, moderate and high risk, respectively, of CVD and myocardial infraction.7 Therefore, blood CRP level has become a promising measure of CVD risk.8-9
Native protein purified from human fluids
Immunogen grade antigen for research use or manufacturing only.
DATA SHEET
FORMULATION
Solution in 0.1M Tris, 0.2M NaCl, 2mM CaCl2, 0.1% NaN3, PH:7.5
APPLICATION/ USAGE
Standard ELISA test, Western blotting
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS-PAGE to determine purity of the protein.
STORAGE
Less than 3 months: store at 4°C.
For long-term storage: aliquot and freeze at -20°C. Avoid repeated freeze/defrost cycles.
REFERENCE
1- Thompson D., Pepys M.B. and Wood S.P. (1999) Structure, 7, 169-177.
2- Festa A, D’Agostino R. Jr., Tracy R.P. and Haffner S.M. (2002) Diabetes, 51, 1131-1137.
3- Verma S. and Yeh E.T. (2003) Am J Physiol, 285, R1253-R1258.
4- Jialal I., Devaraj S. and Venugopal S.K. (2004) Hypertension, 44, 6-11.
5- Kindmark C.O. (1972) Scand J Clin Lab Invest, 29, 407-411.
6- Macy E.M., Hayes T.E. and Tracy R.P. (1997) Clin Chem, 43, 52-58.
7- Ridker P.M. (2004) Am Heart Hosp J, 2 (4 Suppl 1), 4-9.
8- Benzaquen l.R., Yu H. and Rifai N. (2002) Crit Rev Clin Lab Sci, 39, 459-497.
9- Pearson T.A. et al., (2003) Circulation, 107, 499-511.