Cystatin C Turbidimetric Immunoassay Kit

Cystatin C Turbidimetric Immunoassay Kit

SKU: 51240
  • INTRODUCTION

    Human cystatin C (or cystatin 3), which is composed of 120 amino acid residues, belongs to the cystatins superfamily that inactivates lysosomal cysteine proteinases. As a strongly cationic and low-molecular weight (13.4 kDa) protein, it is almost freely filtered across the glomerular membrane, and is therefore used as a biomarker of kidney function. A growing body of evidence suggests that cystatin C is a more reliable biomarker of glomerular filtration rate than creatinine. The normal range of Cystatin C in plasma sample is 0.5 – 10.3 mg/L. In addition to kidney disease, altered serum levels of cystatin C are associated with several types of cardiovascular disease, including myocardial infarction, stroke, heart failure, peripheral arterial disease and metabolic syndrome.

    IMD developed its cystatin C PETIA kit with excellent reproducibility and accuracy equivalent to FDA-approved assays.

  • PRINCIPLE OF THE ASSAY

    This assay is a turbidimetric immunoassay for the quantitative measurement of Cystatin C in human serum, plasma and urine.  A standard or sample is added into a cuvette and mixed with the reaction buffer R1. After a short incubation, the test reagent R2, which is a suspension of microparticles coated with Cystatin C antibodies, is added into the cuvette and mixed. The presence of Cystatin C in the standard or sample causes the immune-particles to aggregate. The extent to which the microparticles aggregate is quantified by the amount of light scattering measured as absorbance by a chemistry analyzer. The concentration of Cystatin C in unknown samples can be interpolated from a reference curve using the standards provided.

  • PERFORMANCE OF THE KIT

    TYPICAL STANDARD CURVE

    Cystatin C  (mg/L)

    Absorbance (570 nm)

    0

    0.0072

    0.5

    0.0455

    1

    0.0920

    2

    0.1953

    4

    0.3395

    8

    0.4547

  • ASSAY PROCEDURE

    Assay procedures may vary depending on the automated chemistry analyzer to be used. A general example of assay procedures is stated as follow:

    1. Dispense 150µl of R1 into a clean cuvette

    2. Add 1.5µl of sample and incubate at 37˚C for 5 minutes

    3. Further add 50µl of R2

    4. Read change of absorbance at 570 nm for 8 minutes after the addition of R2

    5. Calculate the concentration of Cystatin C in unknown sample by interpolation from a reference curve using the standards provided

  • PACKING SPECIFICATIONS

    Cat. No.

    Size

    Approximately tests

    51240 -05

    R1: 15ml, R2: 5ml

    100

    51240 -10

    R1: 30ml, R2: 10ml

    200

    51240 -20

    R1: 60ml, R2: 20ml

    400

    51240 -50

    R1: 150ml, R2: 50ml

    1000

    51240 -100

    R1: 300ml, R2: 100ml

    2000

    For bulk order, please enquire

  • OTHER MATERIALS REQUIRED

    Product           

    Catalog           Number

    Format

    Method     

    Calibrator                 

    51240-S1

    5 x 1ml

     

    Control

    51240-C1

    2 x 1ml

     

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