GAD65-45tr (Human Glutamic Acid Decarboxylase 65kDa Isoform, 1-45aa truncated)

GAD65-45tr (Human Glutamic Acid Decarboxylase 65kDa Isoform, 1-45aa truncated)

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Origin:

Recombinant

Cat. No.:

41846

Tag:

N-terminal 6xHis

Size:

0.1 mg

Source:

Spodopterafrugiperda Sf9

Purity:

>95%

Species:

Human

Abbreviation

GAD65(1-45tr)

  • INTRODUCTION

    GAD65 is primarily expressed in neuron cells and pancreas β-cells. It works as a catalyser in GABA synthesis. 

    Glutamic acid decarboxylase autoantibodies (GADA) are found in 70% to 80% of individuals with new-onset type 1 diabetes, making it the most frequent autoantibody in autoimmune diabetes. GADA can be detected in serum for many years post diagnosis, and high concentrations of GADA have been considered as a marker of faster β-cell exhaustion in these patients. Furthermore, GADA in non-diabetic individuals predicts the later development of type 1 diabetes1. Besides autoimmune diabetes, GADA also exists in Stiff Man Syndrome, autoimmune poly-endocrinopathies, and some of Grave’s Disease patients.

  • DATA SHEET

  • DESCRIPTION

    Expressed in Baculovirus-sf9 vector expression system with total 566 amino acids (AA).

    Mw: 63.9kDa (calculated).

    N-terminal 6xHis-tag and TEV cleavage site, 25 extra AA (highlighted).

    Recombinant antigen for research use or manufacturing only.

  • AMINO ACID SEQUENCE

    MSYYHHHHHHDYDIPTTENLYFQGAALLYGDAEKPAESGGSQPPRAAARKAACACDQKPCSCSKVDVNYAFLHATDLLPACDGERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWELADQPQNLEEILMHCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWLTSTANTNMFTYEIAPVFVLLEYVTLKKMREIIGWPGGSGDGIFSPGGAISNMYAMMIARFKMFPEVKEKGMAALPRLIAFTSEHSHFSLKKGAAALGIGTDSVILIKCDERGKMIPSDLERRILEAKQKGFVPFLVSATAGTTVYGAFDPLLAVADICKKYKIWMHVDAAWGGGLLMSRKHKWKLSGVERANSVTWNPHKMMGVPLQCSALLVREEGLMQNCNQMHASYLFQQDKHYDLSYDTGDKALQCGRHVDVFKLWLMWRAKGTTGFEAHVDKCLELAEYLYNIIKNREGYEMVFDGKPQHTNVCFWYIPPSLRTLEDNEERMSRLSKVAPVIKARMMEYGTTMVSYQPLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL

  • STORAGE

    Store at –80°C.  Avoid repeated freezing/thawing cycles.

  • RECONSTITUTION

    Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.

     

  • QUALITY CONTROL TEST

    BCA to determine quantity of the protein.

    SDS PAGE to determine purity of the protein.

  • FORMULATION

    Liquid in Phosphate buffer containing NaCl (1M), Na2HPO4 (10mM), KCl (2.7mM) and KH2PO4 (2.0mM) with protease inhibitor, pH8.0.

  • APPLICATION

    Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.

  • SDS-PAGE Gel

    See image

  • IMMUNOLOGICAL FUNCTION

    As an autoantigen, GAD65 binds with IgG-type human autoantibodies.

$265.00Price

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