GAD65-fl (Human Glutamic Acid Decarboxylase 65kDa Isoform, Full-length)
Origin:
Recombinant
Cat. No.:
41842
Tag:
N-terminal 6xHis
Size:
0.1 mg
Source:
Spodopterafrugiperda Sf9
Purity:
>95%
Species:
Human
Abbreviation
GAD65-fl
INTRODUCTION
GAD65 is primarily expressed in neuron cells and pancreas β-cells. It works as a catalyser in GABA synthesis.
Glutamic acid decarboxylase autoantibodies (GADA) are found in 70% to 80% of individuals with new-onset type 1 diabetes, making it the most frequent autoantibody in autoimmune diabetes. GADA can be detected in serum for many years post diagnosis, and high concentrations of GADA have been considered as a marker of faster β-cell exhaustion in these patients. Furthermore, GADA in non-diabetic individuals predicts the later development of type 1 diabetes1. Besides autoimmune diabetes, GADA also exists in Stiff Man Syndrome, autoimmune poly-endocrinopathies, and some of Grave’s Disease patients.
DESCRIPTION
Expressed in Baculovirus-sf9 vector expression system with total 612 amino acids (AA).
Mw: 67.65 KDa (calculated).
N-terminal 6xHis-tag and TEV cleavage site, 25 extra AA (highlighted).
Recombinant antigen for research use or manufacturing only.
AMINO ACID SEQUENCE
MSYYHHHHHHDYDIPTTENLYFQGAASPGSGFWSFGSEDGSGDSENPGTARAWCQVAQKFTGGIGNKLCALLYGDAEKPAESGGSQPPRAAARKAACACDQKPCSCSKVDVNYAFLHATDLLPACDGERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWELADQPQNLEEILMHCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWLTSTANTNMFTYEIAPVFVLLEYVTLKKMREIIGWPGGSGDGIFSPGGAISNMYAMMIARFKMFPEVKEKGMAALPRLIAFTSEHSHFSLKKGAAALGIGTDSVILIKCDERGKMIPSDLERRILEAKQKGFVPFLVSATAGTTVYGAFDPLLAVADICKKYKIWMHVDAAWGGGLLMSRKHKWKLSGVERANSVTWNPHKMMGVPLQCSALLVREEGLMQNCNQMHASYLFQQDKHYDLSYDTGDKALQCGRHVDVFKLWLMWRAKGTTGFEAHVDKCLELAEYLYNIIKNREGYEMVFDGKPQHTNVCFWYIPPSLRTLEDNEERMSRLSKVAPVIKARMMEYGTTMVSYQPLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDLSR
STORAGE
Store at –80°C. Avoid repeated freezing/thawing cycles.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
FORMULATION
Liquid in Phosphate buffer containing NaCl (1M), Na2HPO4 (10mM), KCl (2.7mM) and KH2PO4 (2.0mM) with protease inhibitor, pH8.0.
APPLICATION
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.
IMMUNOLOGICAL FUNCTION
As an autoantigen, GAD65 binds with IgG-type human autoantibodies.