Zinc Transporter-8 (ZnT8) Autoantibody ELISA kit from ImmunoDiagnostics
Kit Size: 96 wells/Kit
INTRODUCTION
ZnT8 autoantibodies are important serological markers for diagnosing type 1 diabetes mellitus (T1D). ZnT8 is found in pancreatic beta cells and plays a role in regulating insulin secretion. ZnT8 autoantibodies primarily target the C-terminal domain of ZnT8 (residues 268-369). In
the human population, gene polymorphism at the codon for the 325th amino acid leads to the expression of three protein variants: Arginine (R) 325, Tryptophan (W) 325, and rarely Glutamine (Q) 325. ZnT8 Ab can be specific to the R325 or W325 variant or non-specific to residue 325. Sera that reacts exclusively with the Q allele is extremely uncommon The IMD ZnT8 Ab ELISA is capable of detecting and quantifying autoantibodies specific to R325, W325, or non-specific to residue 325 variants.ASSAY CHARACTERISTICS
Clinical Evaluation
Sensitivity
48.72% (n=40)
Specificity
100% (n=77)
Inter-Assay Precision
Sample
IU/ml (n=6)
CV
1
450
5.30%
2
40
2.34%
3
10
4.38%
Intra-Assay Precision
Sample
IU/ml (n=20)
CV
1
450
5.46%
2
40
6.31%
3
10
3.2%
Sample Type: SerumStorage : 4°C
References:
- Yang, Lin, et al. "The diagnostic value of zinc transporter 8 autoantibody (ZnT8A) for type 1 diabetes in Chinese." Diabetes/metabolism research and reviews 26.7 (2010): 579-584.
- Parsons, Douglas S., Christer Hogstrand, and Wolfgang Maret. "The C‐terminal cytosolic domain of the human zinc transporter ZnT8 and its diabetes risk variant." The FEBS Journal 285.7 (2018): 1237-1250.