Autoantibodies against islet cell antigens, such as glutamic acid decarboxylase

(GAD), insulinoma-associated antigen 2 (IA-2), insulin and zinc transporter 8 (ZnT8), are important serological biomarkers for the accurate classification and diagnosis of autoimmune diabetes mellitus. Detection of GAD autoantibodies (GADA) and IA2-2 autoantibodies (IA2A) are included into the algorithm of autoimmune diabetes diagnosis. Combined detection of IA2A and GADA can lead to a 94% detection rate for the diagnosis of autoimmune diabetes. IMD GADA and IA2A Duo Islet autoantibodies detection ELISA is able to detect GADA and IA2A simultaneously in the same serum sample.

Clinical Evaluation

Sensitivity	94.8% (n=40)
Specificity	98.21% (n=77)

 

Inter-Assay Precision

 

Sample	IU/ml (n=6)	CV
1	140	3.82%
2	40	5.5%
3	4	4.22%

 

Intra-Assay Precision

Sample	IU/ml (n=20)	CV
1	140	3.10%
2	40	5.23%
3	4	4.85%

Sample Type: Serum 

Storage : 4°C

1. Yang, Lin, et al. "The diagnostic value of zinc transporter 8 autoantibody (ZnT8A) for type 1 diabetes in Chinese." Diabetes/metabolism research and reviews 26.7 (2010): 579-584.
2. Parsons, Douglas S., Christer Hogstrand, and Wolfgang Maret. "The C‐terminal cytosolic domain of the human zinc transporter ZnT8 and its diabetes risk variant." The FEBS Journal 285.7 (2018): 1237-1250.