©2019 by ImmunoDiagnostics Limited.

Histone H2A

 

Origin: Recombinant

Cat. No.: 41810

Tag: N-terminal 6xHis

Size: 0.1 mg

Source: E.coli

Purity: >90%

Other Names: H2A

Species: Human

  • INTRODUCTION

    Histones are proteins that package DNA into nucleosomes and are responsible for maintaining the shape and structure of a nucleosome. 

    There are five families of histones known to date, H1/H5, H2A, H2B, H3, and H4. H2A, along with H2B, H3 and H4, is considered as a core histone. 

    Anti-histone autoantibodies are found in 50%-70% of patients with systemic lupus erythematosus (SLE) and in more than 95% of patients with drug-induced lupus erythematosus.

  • DATA SHEET

  • IMMUNOLOGICAL FUNCTION

    As an autoantigen, H2A binds with IgG-type human auto-antibodies.

  • AMINO ACID SEQUENCE

    METSYYHHHH HHDYDIPTTE NLYFQGASGR GKQGGKARAK AKSRSSRAGL QFPVGRVHRL
    LRKGNYSERV GAGAPVYLAA VLEYLTAEIL ELAGNAARDN KKTRIIPRHL QLAIRNDEEL
    NKLLGRVTIA QGGVLPNIQA VLLPKKTESH HKAKGK

  • APPLICATIONS

    Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.

  • FORMULATION

    Lyophilized at 1 mg/mL in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, PH8.0).

  • RECONSTITUTION

    Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let thelyophilized pellet dissolve completely.

  • DESCRIPTION

    Expressed in E.coli cells with total 156 AA. Mw: 17.3 KDa (calculated). 

    N-terminal 6xHis-tag and TEV cleavage site, 27 extra AA (highlighted).

    Recombinant antigen for research use or manufacturing only.

  • STORAGE

    Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C.  Avoid repeatedfreezing /thawing cycles.

  • QUALITY CONTROL TEST

    BCA to determine quantity of the protein.

    SDS PAGE to determine purity of the protein.

  • SDS-PAGE Gel

    Refer to picture 

$998.00Price
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