Histones are proteins that package DNA into nucleosomes and are responsible for maintaining the shape and structure of a nucleosome.
There are five families of histones known to date, H1/H5, H2A, H2B, H3, and H4. H2A, along with H2B, H3 and H4, is considered as a core histone.
Anti-histone autoantibodies are found in 50%-70% of patients with systemic lupus erythematosus (SLE) and in more than 95% of patients with drug-induced lupus erythematosus.
As an autoantigen, H2A binds with IgG-type human auto-antibodies.
AMINO ACID SEQUENCE
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.
Lyophilized in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, PH8.0).
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let thelyophilized pellet dissolve completely.
Expressed in E.coli cells with a total of 173 AA.
Mw: 19.0 KDa (calculated).
N-terminal 6xHis-tag, Xpress Epitope, EK recognition site, and TEV cleavage site, 44 extra AA (highlighted).
Recombinant antigen for research use or manufacturing only.
Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C. Avoid repeatedfreezing /thawing cycles.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Refer to picture