©2019 by ImmunoDiagnostics Limited.

Human Annexin

SKU: 41720

Origin:

Recombinant

Cat. No.:

41720

Tag:

N-terminal 6xHis

Size:

0.1 mg

Source:

E.coli

Purity:

>90%

Other Names:

ANXA8

Species:

Human

  • INTRODUCTION

    Annexin A8 (ANXA8) belongs to the annexin family of evolutionally conversed Ca2+ and phospholipid binding proteins. ANXA8 may function as an anticoagulant which inhibits the function of thromboplastin-specific complex in an indirect way. Overexpression of this gene has been shown to be associated with acute myelocytic leukemia.

    Human ANXA8 shares 92.661% sequence identity with rat ANXA8 and 92.094% with mouse ANXA8.

  • DATA SHEET

  • DESCRIPTION

    Expressed in E.coli cells with total 392 AA. Mw: 44.35 kDa (calculated). 

    N-terminal 6xHis-tag, EK and TEV cleavage site, 46 extra AA (highlighted).

    Recombinant antigen for research use or manufacturing only.

  • AMINO ACID SEQUENCE

    MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSLEEN LYFQGAAWWK SWIEQEGVTV
    KSSSHFNPDP DAETLYKAME TKGIGTNEQA IIDVLTKRSN TQRQQIAKSF KAQFGKDLTE
    TLKSELSGKF ERLIVALMET YPPYRYEAKE LHDAMETKGL GTKEGVIIEI LASRTKNQLR
    EIMETKAYEE DYGSSLEEDI QADTSGYLER ILVCLLQGSR DDVSSFVDPG LALQDAQDLY
    AAGEKIRGTD EMETKFITIL CTRSATHLLR VFEEYEKIAN KSIEDSIKSE THGSLEEAME
    TLTVVKCTQN LHSYFAERLY YAMETKGAGT RDGTLIRNIV SRSEIDLNLI KCHFKKMETY
    GKTLSSMETI METEDTSGDY KNALLSLVGS DP

  • STORAGE

    Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C.  Avoid repeated freezing/thawing cycles.

  • RECONSTITUTION

    Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.

  • QUALITY CONTROL TEST

    BCA to determine quantity of the protein.

    SDS PAGE to determine purity of the protein.

  • FORMULATION

    Lyophilized at 1 mg/mL in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, PH8.0).

  • APPLICATION

    ELISA and Western Blotting.

  • SDS-PAGE Gel

    Lyophilized at 1 mg/mL in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, PH8.0).

  • ENDOTOXIN LEVEL

    <0.2 EU per 1 μg of the protein by the LAL method.

$200.00Price