Human Annexin A8 (ANXA8)
Annexin A8 (ANXA8) belongs to the annexin family of evolutionally conversed Ca2+ and phospholipid binding proteins. ANXA8 may function as an anticoagulant which inhibits the function of thromboplastin- specific complex in an indirect way. Overexpression of this gene has been shown to be associated with acute myelocytic leukemia. Human ANXA8 shares 92.661% sequence identity with rat ANXA8 and 92.094% with mouse ANXA8.
Expressed in E.coli cells with total 392 AA. Mw: 44.35 KDa (calculated).
N-terminal 6xHis-tag, EK and TEV cleavage site, 44 extra AA (highlighted).
Recombinant antigen for research use or manufacturing only.
AMINO ACID SEQUENCE
Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C. Avoid repeated freezing/thawing cycles.
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Lyophilized at 1 mg/mL in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, PH8.0).
ELISA and Western Blotting.
<0.2 EU per 1 μg of the protein by the LAL method.