Human Anti-dsDNA ELISA (IgG) Kit

Human Anti-dsDNA ELISA (IgG) Kit

SKU: 31A040

★ Download Datasheet PDF ★

 

Kit Size: 96 wells/Kit

 

Typical results

(Example only, not for calculation of actual results)

Calibrator*

Concentration (IU/mL)

OD450 (Blanked)

1

600

1.149

2

400

0.809

3

200

0.594

4

100

0.350

5

80

0.265

6

30

0.180

7

20

0.163

Negative Control

0

0.049

Positive

Control

210

0.538

 

*Calibrator standardised against International Standard WHO Wo/80.

 

 

Cut-off

IU/mL

Negative

< 25 IU/mL

Positive

≧ 25 IU/mL

*This cut off has been validated at ImmunoDiagnostics. However, we recommend each laboratory establish its own reference ranges of anti-dsDNA levels to distinguish healthy and pathological subjects, as well as include its own internal control samples in the assay.

 

  • INTRODUCTION

    The dsDNA-specific autoantibodies (anti-dsDNA) are considered a specific marker for systemic lupus erythematosus (SLE), due to the high clinical associations.

    The presence of anti-dsDNA autoantibodies in other pathological conditions and in healthy subjects is very rare (< 0.5%). In contrast, they may be present in SLE patients even before they develop clinical symptoms.

    Therefore, regular measurement of anti-dsDNA autoantibodies in blood is important for early diagnosis, as well as monitoring the progression of SLE.

     

  • ASSAY CHARACTERISTICS

    Clinical Evaluation

    Sensitivity

    79.69% (n=64)

    Specificity

    96.77% (n=40)

     

    Inter-Assay Precision

    Sample

    IU/mL (n=20)

    CV

    1

    394

    2.6%

    2

    144

    8.4%

    3

    81

    9.4%

     

    Intra-Assay Precision

    Sample

    IU/mL (n=20)

    CV

    1

    375

    2.5%

    2

    120

    8.4%

    3

    73

    9.3%

     

    Sample Type: Serum and plasma

    Storage : 4°C

  • References:

    1.D. A. Isenberg, et al. Fifty Years Of Anti-ds Dna Antibodies: Are We Approaching Journey's End? Rheumatology, Vol. 46, No. 7, Pp. 1052–1056, 2007.

    2.G. C. Tsokos. Systemic Lupus Erythematosus. The New England Journal Of Medicine, Vol. 365, No. 22, Pp. 2110–2121, 2011.

    3.E. Cozzani, et al. Serology Of Lupus Erythematosus: Correlation Between Immunopathological Features And Clinical Aspects,” Autoimmune Diseases, Vol. 2014, Article Id 321359, 13 Pages, 2014.

  • DATA SHEET

$400.00Price

For bulk order (QTY: 5 or more), please contact us for quote

Featured Products