Human Anti-dsDNA ELISA (IgG) Kit
  • Human Anti-dsDNA ELISA (IgG) Kit

    ★ Download Datasheet PDF ★

     

    Typical results

    (Example only, not for calculation of actual results)

    Calibrator*

    Concentration (IU/mL)

    OD450 (Blanked)

    1

    600

    1.149

    2

    400

    0.809

    3

    200

    0.594

    4

    100

    0.350

    5

    80

    0.265

    6

    30

    0.180

    7

    20

    0.163

    Negative Control

    0

    0.049

    Positive

    Control

    210

    0.538

     

    *Calibrator standardised against International Standard WHO Wo/80.

     

     

    Cut-off

    IU/mL

    Negative

    < 25 IU/mL

    Positive

    ≧ 25 IU/mL

    *This cut off has been validated at ImmunoDiagnostics. However, we recommend each laboratory establish its own reference ranges of anti-dsDNA levels to distinguish healthy and pathological subjects, as well as include its own internal control samples in the assay.

     

    • INTRODUCTION

      The dsDNA-specific autoantibodies (anti-dsDNA) are considered a specific marker for systemic lupus erythematosus (SLE), due to the high clinical associations.

      The presence of anti-dsDNA autoantibodies in other pathological conditions and in healthy subjects is very rare (< 0.5%). In contrast, they may be present in SLE patients even before they develop clinical symptoms.

      Therefore, regular measurement of anti-dsDNA autoantibodies in blood is important for early diagnosis, as well as monitoring the progression of SLE.

       

    • ASSAY CHARACTERISTICS

      Clinical Evaluation

      Sensitivity

      79.69% (n=64)

      Specificity

      96.77% (n=40)

       

      Inter-Assay Precision

      Sample

      IU/mL (n=20)

      CV

      1

      394

      2.6%

      2

      144

      8.4%

      3

      81

      9.4%

       

      Intra-Assay Precision

      Sample

      IU/mL (n=20)

      CV

      1

      375

      2.5%

      2

      120

      8.4%

      3

      73

      9.3%

       

      Sample Type: Serum and plasma

      Storage : 4°C

    • References:

      1.D. A. Isenberg, et al. Fifty Years Of Anti-ds Dna Antibodies: Are We Approaching Journey's End? Rheumatology, Vol. 46, No. 7, Pp. 1052–1056, 2007.

      2.G. C. Tsokos. Systemic Lupus Erythematosus. The New England Journal Of Medicine, Vol. 365, No. 22, Pp. 2110–2121, 2011.

      3.E. Cozzani, et al. Serology Of Lupus Erythematosus: Correlation Between Immunopathological Features And Clinical Aspects,” Autoimmune Diseases, Vol. 2014, Article Id 321359, 13 Pages, 2014.

    • DATA SHEET

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