Human Anti-Histones ELISA (IgG)
Histones are cationic proteins which associate with DNA in the nucleus of eukaryotic cells to form nucleosomes. Anti-histone antibodies occur in a number of clinical conditions, primarily in systemic lupus erythematosus (SLE) and drug-induced lupus (DIL), and also in other systemic and organ-specific autoimmune diseases, and certain neurological and infectious diseases. Anti-histone antibodies are found in up to 80% of SLE patients, and 95% of the cases with DIL by procainamide, hydralazine, chlorpromazine, and quinidine. Despite of SLE and DIL, anti-histone antibodies are commonly seen in other rheumatic diseases, including myositis and systemic sclerosis (SSc). Therefore, anti-histone antibodies are a common biomarker for evaluating the autoimmune diseases.
The linearity of Anti-dsDNA ELISA (IgG) was determined by assaying 8 serial dilutions of 5 serum samples. The linear regreassion was calculated, calculated, R2 amounting to >0.98 within the concentration range of 10 RU/mL to 300 RU/mL.
The reproducibility of the test was investigated by determine the intra- and inter-assay coefficients of variation using 3 sera. The Intra-assay CVs are based on 20 determinations and the inter-assay CVs on 4 determinations performed in 6 different plates.
- DUMORTIER, H., & MULLER, S. (2007). Histone autoantibodies. In Autoantibodies (pp. 169-176). Elsevier.
- Dooley, M. A. (2016). Drug-induced lupus. In Systemic Lupus Erythematosus (pp. 473-479). Academic Press.
- Firestein, G. S., Budd, R., Gabriel, S. E., McInnes, I. B., & O'Dell, J. R. (2016). Kelley and Firestein's Textbook of Rheumatology E-Book. Elsevier Health Sciences.
- Portanova, J. P., Arndt, R. E., Tan, E. M., & Kotzin, B. L. (1987). Anti-histone antibodies in idiopathic and drug-induced lupus recognize distinct intrahistone regions. The Journal of Immunology, 138(2), 446-451.
- Portanova, J. P., Rubin, R. L., Joslin, F. G., Agnello, V. D., & Tan, E. M. (1982). Reactivity of anti-histone antibodies induced by procainamide and hydralazine. Clinical immunology and immunopathology, 25(1), 67-79.