Human Anti-Histones (IgG) ELISA Kit

Human Anti-Histones (IgG) ELISA Kit

SKU: 31A030

★ Download Datasheet PDF ★

 

Kit Size: 96 wells/kit

 

Typical results

(Example only, not for calculation of actual results)

Calibrator*

Concentration (RU/mL)

OD450 (Blanked)

1

500

1.469

2

300

0.895

3

200

0.629

4

100

0.444

5

50

0.209

6

30

0.136

7

10

0.053

Negative Control

0

0.054

Positive

Control

147

0.480

 

* Calibrator standardised against NIBSC W1064. (RU=Relative Unit)

 

Cut-off

RU/mL

Negative

< 42 RU/mL

Positive

≧ 42 RU/mL

 

*This cut off has been validated at ImmunoDiagnostics. However, we recommend each laboratory establish its own reference ranges of anti-histones levels to distinguish healthy and pathological subjects, as well as include its own internal control samples in the assay.

 

  • INTRODUCTION

    Histones are DNA-associated cationic proteins in the nucleus of eukaryotic cells to form nucleosomes.

    Anti-histone autoantibodies are present in a number of clinical conditions, primarily in systemic lupus erythematosus (SLE, ~80%) and drug-induced lupus (DIL, ~95%) by procainamide, hydralazine, chlorpromazine, and quinidine.

    Additionally, these autoantibodies are also detectable in other rheumatic diseases, including myositis and systemic sclerosis (SSc).

     

  • ASSAY CHARACTERISTICS

    Clinical Evaluation

    Sensitivity

    85.29% (n=34)

    Specificity

    100% (n=40)


    Inter-Assay Precision

    Sample

    RU/mL (n=20)

    CV

    1

    64

    9.0%

    2

    173

    8.1%

    3

    363

    7.5%

     

    Intra-Assay Precision

    Sample

    RU/mL (n=20)

    CV

    1

    83

    4.0%

    2

    222

    4.6%

    3

    419

    3.1%


    Sample Type: Serum and plasma

    Storage : 4°C

  • References:

    1.Dumortier, H., & Muller, S. (2007). Histone Autoantibodies. In Autoantibodies (pp. 169-176). Elsevier.

    2.Dooley, M. A. (2016). Drug-induced Lupus. In Systemic Lupus Erythematosus (pp. 473-479). Academic Press.

    3.Firestein, G. S., Budd, R., Gabriel, S. E., Mcinnes, I. B., & O'dell, J. R. (2016). Kelley And Firestein's Textbook Of Rheumatology E-book. Elsevier Health Sciences.

    4.Portanova, J. P., Arndt, R. E., Tan, E. M., & Kotzin, B. L. (1987). Anti-histone Antibodies In Idiopathic And Drug-induced Lupus Recognize Distinct Intrahistone Regions. The Journal Of Immunology, 138(2), 446-451.

    5.Portanova, J. P., Rubin, R. L., Joslin, F. G., Agnello, V. D., & Tan, E. M. (1982). Reactivity Of Anti-histone Antibodies Induced By Procainamide And Hydralazine. Clinical Immunology And Immunopathology, 25(1), 67-79.

  • DATA SHEET

$400.00Price

For bulk order (QTY: 5 or more), please contact us for quote