Human Anti-Histones (IgG) ELISA Kit
Kit Size: 96 wells/kit
Typical results
(Example only, not for calculation of actual results)
Calibrator* | Concentration (RU/mL) | OD450 (Blanked) |
1 | 500 | 1.469 |
2 | 300 | 0.895 |
3 | 200 | 0.629 |
4 | 100 | 0.444 |
5 | 50 | 0.209 |
6 | 30 | 0.136 |
7 | 10 | 0.053 |
Negative Control | 0 | 0.054 |
Positive Control | 147 | 0.480 |
* Calibrator standardised against NIBSC W1064. (RU=Relative Unit)
Cut-off | RU/mL |
Negative | < 42 RU/mL |
Positive | ≧ 42 RU/mL |
*This cut off has been validated at ImmunoDiagnostics. However, we recommend each laboratory establish its own reference ranges of anti-histones levels to distinguish healthy and pathological subjects, as well as include its own internal control samples in the assay.
INTRODUCTION
Histones are DNA-associated cationic proteins in the nucleus of eukaryotic cells to form nucleosomes.
Anti-histone autoantibodies are present in a number of clinical conditions, primarily in systemic lupus erythematosus (SLE, ~80%) and drug-induced lupus (DIL, ~95%) by procainamide, hydralazine, chlorpromazine, and quinidine.
Additionally, these autoantibodies are also detectable in other rheumatic diseases, including myositis and systemic sclerosis (SSc).
ASSAY CHARACTERISTICS
Clinical Evaluation
Sensitivity
85.29% (n=34)
Specificity
100% (n=40)
Inter-Assay PrecisionSample
RU/mL (n=20)
CV
1
64
9.0%
2
173
8.1%
3
363
7.5%
Intra-Assay Precision
Sample
RU/mL (n=20)
CV
1
83
4.0%
2
222
4.6%
3
419
3.1%
Sample Type: Serum and plasmaStorage : 4°C
References:
1.Dumortier, H., & Muller, S. (2007). Histone Autoantibodies. In Autoantibodies (pp. 169-176). Elsevier.
2.Dooley, M. A. (2016). Drug-induced Lupus. In Systemic Lupus Erythematosus (pp. 473-479). Academic Press.
3.Firestein, G. S., Budd, R., Gabriel, S. E., Mcinnes, I. B., & O'dell, J. R. (2016). Kelley And Firestein's Textbook Of Rheumatology E-book. Elsevier Health Sciences.
4.Portanova, J. P., Arndt, R. E., Tan, E. M., & Kotzin, B. L. (1987). Anti-histone Antibodies In Idiopathic And Drug-induced Lupus Recognize Distinct Intrahistone Regions. The Journal Of Immunology, 138(2), 446-451.
5.Portanova, J. P., Rubin, R. L., Joslin, F. G., Agnello, V. D., & Tan, E. M. (1982). Reactivity Of Anti-histone Antibodies Induced By Procainamide And Hydralazine. Clinical Immunology And Immunopathology, 25(1), 67-79.
DATA SHEET