Human Anti-Histones (IgG) ELISA Kit
Kit Size: 96 wells/kit
(Example only, not for calculation of actual results)
* Calibrator standardised against NIBSC W1064. (RU=Relative Unit)
< 42 RU/mL
≧ 42 RU/mL
*This cut off has been validated at ImmunoDiagnostics. However, we recommend each laboratory establish its own reference ranges of anti-histones levels to distinguish healthy and pathological subjects, as well as include its own internal control samples in the assay.
Histones are DNA-associated cationic proteins in the nucleus of eukaryotic cells to form nucleosomes.
Anti-histone autoantibodies are present in a number of clinical conditions, primarily in systemic lupus erythematosus (SLE, ~80%) and drug-induced lupus (DIL, ~95%) by procainamide, hydralazine, chlorpromazine, and quinidine.
Additionally, these autoantibodies are also detectable in other rheumatic diseases, including myositis and systemic sclerosis (SSc).
Sample Type: Serum and plasma
Storage : 4°C
1.Dumortier, H., & Muller, S. (2007). Histone Autoantibodies. In Autoantibodies (pp. 169-176). Elsevier.
2.Dooley, M. A. (2016). Drug-induced Lupus. In Systemic Lupus Erythematosus (pp. 473-479). Academic Press.
3.Firestein, G. S., Budd, R., Gabriel, S. E., Mcinnes, I. B., & O'dell, J. R. (2016). Kelley And Firestein's Textbook Of Rheumatology E-book. Elsevier Health Sciences.
4.Portanova, J. P., Arndt, R. E., Tan, E. M., & Kotzin, B. L. (1987). Anti-histone Antibodies In Idiopathic And Drug-induced Lupus Recognize Distinct Intrahistone Regions. The Journal Of Immunology, 138(2), 446-451.
5.Portanova, J. P., Rubin, R. L., Joslin, F. G., Agnello, V. D., & Tan, E. M. (1982). Reactivity Of Anti-histone Antibodies Induced By Procainamide And Hydralazine. Clinical Immunology And Immunopathology, 25(1), 67-79.