Human Anti-Histones (IgG) ELISA Kit
  • Human Anti-Histones (IgG) ELISA Kit

    ★ Download Datasheet PDF ★

     

    Typical results

    (Example only, not for calculation of actual results)

    Calibrator*

    Concentration (RU/mL)

    OD450 (Blanked)

    1

    500

    1.469

    2

    300

    0.895

    3

    200

    0.629

    4

    100

    0.444

    5

    50

    0.209

    6

    30

    0.136

    7

    10

    0.053

    Negative Control

    0

    0.054

    Positive

    Control

    147

    0.480

     

    * Calibrator standardised against NIBSC W1064. (RU=Relative Unit)

     

    Cut-off

    RU/mL

    Negative

    < 42 RU/mL

    Positive

    ≧ 42 RU/mL

     

    *This cut off has been validated at ImmunoDiagnostics. However, we recommend each laboratory establish its own reference ranges of anti-histones levels to distinguish healthy and pathological subjects, as well as include its own internal control samples in the assay.

     

    • INTRODUCTION

      Histones are DNA-associated cationic proteins in the nucleus of eukaryotic cells to form nucleosomes.

      Anti-histone autoantibodies are present in a number of clinical conditions, primarily in systemic lupus erythematosus (SLE, ~80%) and drug-induced lupus (DIL, ~95%) by procainamide, hydralazine, chlorpromazine, and quinidine.

      Additionally, these autoantibodies are also detectable in other rheumatic diseases, including myositis and systemic sclerosis (SSc).

       

    • ASSAY CHARACTERISTICS

      Clinical Evaluation

      Sensitivity

      85.29% (n=34)

      Specificity

      100% (n=40)


      Inter-Assay Precision

      Sample

      RU/mL (n=20)

      CV

      1

      64

      9.0%

      2

      173

      8.1%

      3

      363

      7.5%

       

      Intra-Assay Precision

      Sample

      RU/mL (n=20)

      CV

      1

      83

      4.0%

      2

      222

      4.6%

      3

      419

      3.1%


      Sample Type: Serum and plasma

      Storage : 4°C

    • References:

      1.Dumortier, H., & Muller, S. (2007). Histone Autoantibodies. In Autoantibodies (pp. 169-176). Elsevier.

      2.Dooley, M. A. (2016). Drug-induced Lupus. In Systemic Lupus Erythematosus (pp. 473-479). Academic Press.

      3.Firestein, G. S., Budd, R., Gabriel, S. E., Mcinnes, I. B., & O'dell, J. R. (2016). Kelley And Firestein's Textbook Of Rheumatology E-book. Elsevier Health Sciences.

      4.Portanova, J. P., Arndt, R. E., Tan, E. M., & Kotzin, B. L. (1987). Anti-histone Antibodies In Idiopathic And Drug-induced Lupus Recognize Distinct Intrahistone Regions. The Journal Of Immunology, 138(2), 446-451.

      5.Portanova, J. P., Rubin, R. L., Joslin, F. G., Agnello, V. D., & Tan, E. M. (1982). Reactivity Of Anti-histone Antibodies Induced By Procainamide And Hydralazine. Clinical Immunology And Immunopathology, 25(1), 67-79.

    • DATA SHEET

    $330.00Price

    ©2019 by ImmunoDiagnostics Limited.