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High sensitive Human C-reactive Protein ELISA Kit

High sensitive Human C-reactive Protein ELISA Kit

SKU: 31220

 Download Datasheet PDF ★

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Assay range: 0.312 - 20 ng/mL

Kit Size: 96 wells/kit

Other Names: CRP, PTX1


    C-reactive protein (CRP) is a circulating protein mainly secreted from the liver. This acute phase protein consists of five identical non-glycosylated subunits of 23 kDa, that give rise to a symmetrically arranged globular protein with molecular weight of approximately 120 kDa.1 It has long been recognized that CRP is closely related to immunology, inflammation and host defence; as a result it has been used as an inflammatory marker. However, the development of high-sensitivity CRP (hsCRP) ELISA had addressed its role in other clinical issues. There is accumulating evidence suggesting the important role that CRP plays in mediating cardiovascular diseases (CVD) and type 2 diabetes.2-4 Normally CRP is presenting only in a trace amount in circulation (<1 µg/ml)5,6 but can increase over 1,000-fold under acute inflammatory state. Individual with blood CRP levels <1 µg/ml, 1-3 µg/ml and >3 µg/ml is considered to have low, moderate and high risk, respectively, of CVD and myocardial infraction.7 Therefore, blood CRP level has become a promising measure of CVD risk.8,9


    This assay is a quantitative sandwich ELISA using monoclonal antibodies against human CRP. The immunoplate is pre-coated with a monoclonal antibody specific for human CRP. Standards and samples are pipetted into the wells and any human CRP present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase (HRP)-linked monoclonal antibody specific for human CRP is added to the wells. After wash step to remove any unbound reagents, an HRP substrate solution is added and colour develops in proportion to the amount of human CRP bound initially. The assay is stopped and the optical density of the wells determined using a micro-plate reader. Since the increases in absorbance are directly proportional to the amount of captured human CRP, the unknown sample concentration can be interpolated from a reference curve included in each assay.


    A. Typical representation of standard curve

    The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.  

    CRP (ng/ml)

    Absorbance (450 nm)

    Blanked Absorbance


























    B. Sensitivity

    The lowest level of CRP that can be detected by this assay is 0.312 ng/mL.


    C. Specificity

    The antibody pair used in this assay is specific to human CRP and does not cross-react with mouse and rat CRP, and other cytokine or hormone molecules.

    D. Precision

    Intra-assay C.V.: 4.3%

    Inter-assay C.V.: 5.9%


    E. Recovery

    The recovery of the assay was determined by adding various amounts CRP to a sample. The measured concentration of the spiked sample in the assay was compared to the expected concentration. The average recovery was 98%. 


    1. Wu G, Li H, Fang Q, Jiang S, Zhang L, Zhang J, Hou X, Lu J, Bao Y, Xu A, Jia W. Elevated circulating lipocalin-2 levels independently predict incident cardiovascular events in men in a population-based cohort. Arteriosclerosis, thrombosis, and vascular biology. 2014 Nov;34(11):2457-64.
    2. Chen DL, Liess C, Poljak A, Xu A, Zhang J, Thoma C, Trenell M, Milner B, Jenkins AB, Chisholm DJ, Samocha-Bonet D. Phenotypic characterization of insulin-resistant and insulin-sensitive obesity. The Journal of Clinical Endocrinology & Metabolism. 2015 Nov 1;100(11):4082-91.
    3. Chen DL, Brown R, Liess C, Poljak A, Xu A, Zhang J, Trenell M, Jenkins A, Chisholm D, Samocha-Bonet D, Macefield VG. Muscle sympathetic nerve activity is associated with liver insulin sensitivity in obese non-diabetic men. Frontiers in physiology. 2017 Feb 28;8:101.
    4. Yang K, Deng HB, Man AW, Song E, Zhang J, Luo C, Cheung BM, Yuen KY, Jensen PS, Irmukhamedov A, Elie AG. Measuring non‐polyaminated lipocalin‐2 for cardiometabolic risk assessment. ESC heart failure. 2017 Nov;4(4):563-75.
    5. Elie AG, Bloksgaard M, Sun WY, Yang K, Man AW, Xu A, Irmukhamedov A, Riber LP, Wang Y, De Mey JG. Local enrichment of fatty acid-binding protein 4 in the pericardial cavity of cardiovascular disease patients. PloS one. 2018 Nov 5;13(11):e0206802.
    6. Harari A, Coster AC, Jenkins A, Xu A, Greenfield JR, Harats D, Shaish A, Samocha-Bonet D. Obesity and insulin resistance are inversely associated with serum and adipose tissue carotenoid concentrations in adults. The Journal of nutrition. 2020 Jan 1;150(1):38-46.
    7. Liu Y, Wang Y, Ni Y, Cheung CK, Lam KS, Wang Y, Xia Z, Ye D, Guo J, Tse MA, Panagiotou G. Gut microbiome fermentation determines the efficacy of exercise for diabetes prevention. Cell metabolism. 2020 Jan 7;31(1):77-91.
    8. Lui MM, Mak JC, Chong PW, Lam DC, Ip MS. Circulating adipocyte fatty acid–binding protein is reduced by continuous positive airway pressure treatment for obstructive sleep apnea—a randomized controlled study. Sleep and Breathing. 2020 Sep;24(3):817-24.
    9. Lui MM, Tse HF, Lam DC, Lau KK, Chan CW, Ip MS. Continuous positive airway pressure improves blood pressure and serum cardiovascular biomarkers in obstructive sleep apnoea and hypertension. European Respiratory Journal. 2021 Jan 1.
    10. Li D, Li H, Bauer C, Hu Y, Lewis JR, Xu A, Levinger I and Wang Y. Lipocalin-2 variants and their relationship with cardio-renal risk factors. Frontiers in Endocrinology. 2021 Sept 23; 12:781763.
    11. Brzozowska MM, Isaacs M, Bliuc D, Baldock PA, Eisman JA, White CP, Greenfield JR, Center JR. Effects of bariatric surgery and dietary intervention on insulin resistance and appetite hormones over a 3 year period. Sci Rep. 2023 Apr 13;13(1):6032

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