cDNA coding for a minicollagen version of the human collagen IV α3 chain fused to a
hexa-histidine purification tag. The term minicollagen designates the removal of most of
the epitope-less triplehelical collagenous region (situated between the N-terminal 7S
domain and the C-terminal noncollagenous NC1 domain). Expressed in insect cells with
total 280 AA. Mw: 30.6 KDa (calculated).
N-terminal 6xHis-tag and TEV cleavage site, 25 extra AA (highlighted).
Recombinant antigen for research use or manufacturing only.

Goodpasture's disease (GP) is mediated by autoantibodies that bind the glomerular and alveolar basement membrane, causing rapidly progressive glomerulonephritis with or without pulmonary hemorrhage. Type IV collagen, the major structural component of
glomerular basement membranes (GBM). The epitopes recognized by the Goodpasture autoantibodies are sequestered within the NC1 hexamer of the type IV collagen network.
Presence of anti-GBM antibodies confirms the diagnosis of Goodpasture Syndrome

Liquid in 8M Urea

Standard ELISA test, line/dot assay and microarray assay with positive/negative sera
panels.

BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.

Store at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freezing/thawing cycles