Human Cytochrome P450 2D6 (LKM-1)
Origin:
Recombinant
Cat. No.:
41A262
Tag:
N-terminal 6xHis
Size:
0.1 mg
Source:
Spodoptera frugiperda Sf9
Purity:
>80%
Other Names:
LKM-1, CYP2D6
Species:
Human
INTRODUCTION
Cytochrome p450 2D6 is a cytochrome p450 mono-oxygenase that is primarily expressed in the liver and identical with the so-called liver-kidney microsome 1 (LKM 1) antigen. This enzyme localizes to the endoplasmic reticulum and is involved in the detoxification of xenobiotic substances and responsible for the metabolism of many drugs and environmental chemicals that it oxidizes. As the conventional serological repertoire for the diagnosis of AIH, CYP2D6has a prevalence of around 70% in AIH Type 2.
DESCRIPTION
Expressed in insect Sf9 cells with total 521 AA. Mw: 58.7 KDa (calculated).
N-terminal 6xHis-tag and TEV cleavage site, 25 extra AA (highlighted).
Recombinant antigen for research use or manufacturing only.
AMINO ACID SEQUENCE
MSYYHHHHHHDYDIPTTENLYFQGAGLEALVPLAVIVAIFLLLVDLMHRRQRWAARYPPGPLPLPGLGNLLHVDFQNTPYCFDQLRRRFGDVFSLQLAWTPVVVLNGLAAVREALVTHGEDTADRPPVPITQILGFGPRSQGVFLARYGPAWREQRRFSVSTLRNLGLGKKSLEQWVTEEAACLCAAFANHSGRPFRPNGLLDKAVSNVIASLTCGRRFEYDDPRFLRLLDLAQEGLKEESGFLREVLNAVPVLLHIPALAGKVLRFQKAFLTQLDELLTEHRMTWDPAQPPRDLTEAFLAEMEKAKGNPESSFNDENLRIVVADLFSAGMVTTSTTLAWGLLLMILHPDVQRRVQQEIDDVIGQVRRPEMGDQAHMPYTTAVIHEVQRFGDIVPLGVTHMTSRDIEVQGFRIPKGTTLITNLSSVLKDEAVWEKPFRFHPEHFLDAQGHFVKPEAFLPFSAGRRACLGEPLARMELFLFFTSLLQHFSFSVPTGQPRPSHHGVFAFLVSPSPYELCAVPR
STORAGE
Store at –20°C. Avoid repeated freezing/thawing cycles.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Immunodot analysis to determine functionality of protein.
FORMULATION
Liquid in storage buffer (8M Urea, 10mM Tris, 50mM Na2HPO4, 1% SDS, pH8.0).
APPLICATION
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.
IMMUNOLOGICAL FUNCTION
As an autoantigen, CYP2D6 binds with IgG-type human auto-antibodies.