FGF-21, a polypeptide with 210 amino acid residues produced mostly from the liver tissue. Increasing data shows FGF-21 can significantly stimulate glucose uptake in mature adipocytes. FGF-21 exerts its bioactivity through interaction with membrane bound FGF receptors (FGFRs) which requires β-Klotho as a co-factor to bind and activate FGFR signaling. The activation of FGF-21 can induce the stimulation of diverse downstream pathways medicated by MAPK,FRS-2, SHP-2, PI3K, raf, stat and other signaling molecules. In sum, FGF-21 induces a variety of significant beneficial metabolic changes without apparent adverse effects which makes this factor a hot candidate to treat some metabolic diseases.
The Fc-tag can extend the half-life of recombinant proteins in vivo by interacting with the neonatal Fc receptor (FcRn), reducing their clearance from the bloodstream. Therefore, adding an Fc tag to hFGF21 enhances its pharmacokinetic properties in therapeutic protein research.

Lyophilized at 1 mg/mL in NaCl 300mM, KCl 2.7mM, Na2HPO4 10mM, KH2PO4
1.8mM, pH 7.4.

Cell culture, animal studies, ELISA and Western blotting.

BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.

Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C.
Avoid repeated freezing/thawing cycles.

1. Geng L, Yi X, Lin Y, Abulimiti X, Jin L,Yu J, Xu A. Site-specific analysis and functional characterization of N-linked glycosylation for β-Klotho protein. Int J Biol Macromol. 2024 Dec 19:289:138846.