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Human Haptoglobin ELISA kit

SKU: 31400

 

  • INTRODUCTION

    Human haptoglobin is an acute-phase glycoprotein produced predominantly by liver. It is composed of two a subunits (M.W. 16-23 kDa) and two b subunits (M.W. 35-40 kDa). Haptoglobin can bind to free hemoglobin released from lysed erythrocytes and prevent the formation of free radical superoxide that can be formed by the reaction of oxygen and iron from hemoglobin.  It is also known to be involved in immune regulation and anti-inflammation. Elevated amount of haptoglobin is observed during infections and inflammations, obesity, tissue damage etc. Hence, haptoglobin is used as a biomarker to detect acute allograft rejection, proliferative diabetic retinopathy (PDR) and diabetic kidney disease (DKD). Additionally, low haptoglobin levels are mainly observed during hemolytic anemia.

     

  • ASSAY CHARACTERISTICS

    Typical standard curve

    Human         

    haptoglobin

    (ng/mL)

    Absorbance

    (450 nm)

    Blanked Absorbance

    0

    0.087

    0.000

    3.12

    0.135

    0.048

    6.25

    0.194

    0.107

    12.5

    0.347

    0.260

    25

    0.692

    0.605

    50

    1.349

    1.262

    100

    2.650

    2.563

     

    A. Sensitivity: 1.56 ng/mL

    B. Precision: Intra-assay C.V. <5.63%.; Inter-assay C.V <1%.

    C. Spiking: The recovery of human haptoglobin spiked at 5 ng/mL is 104.4%.

    D. Linearity:

    Sample Dilution

    Absorbance

    (450 nm)

    Concentration (ng/mL)

    Recovery (%)

    1:2

    0.601

    5.843

    95.37

    1:4

    0.269

    6.010

    98.10

    1:8

    0.116

    6.790

    110.82

  • References:

    • Andersen, C.B. et al. (2012) Nature 489:456.
    • Shin, A.W. et al.(2014) Am. J. Hematol. 89(4):443-7
    • Körmöczi, G.F. et al. (2016) Eur. J. Clin. Invest. 36(3):202-9.
    • Chiellini, C. et al. (2004) J. Clin. Endocrinol. Metab. 89(6):2678-83.
    • Maffei, M. et al. (2016) Endocr. Rev. 37(4):403-16
    • Yang, J.K. et al. (2017) Diabetes Care 40(2):253-260
  • DATA SHEET

$350.00Price