Human NT-proBNP
Origin: | Recombinant | Cat. No.: | 41660 |
Tag: | N-terminal 6xHis | Size: | 0.1 mg |
Source: | E.coli | Purity: | >95% |
Other names: | Amino terminal brain natriuretic peptide Precursor | Species: | Human |
INTRODUCTION
N-terminal pro-brain (or B-type) natriuretic peptide (NT-proBNP) is produced predominately by the cardiac ventricular myocytes. NT-proBNP is released in response to volume expansion and filling pressure and is involved in maintaining intravascular volume homeostasis. Elevated plasma levels of BNP and NT-proBNP have been observed at times of cardiac stress and damage.
DESCRIPTION
Expressed in E.coli with total 112 AA. Mw: 12.6 KDa (calculated).
N-terminal 6xHis-tag and TEV cleavage site, 36 extra AA (highlighted).
Recombinant antigen for research use or manufacturing only.
AMINO ACID SEQUENCE
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSHPLGSPGSASDLETSGLQEQRNHLQGKLSELQVEQTSLEPLQESPRPTGVWKSREVATEGIRGHRKMVLYTLRAPR
STORAGE
Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C. Avoid repeated freezing/thawing cycles.
RECONSTITUTION
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let thelyophilized pellet dissolves completely.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
FORMULATION
Lyophilized at 1 mg/mL in PBS.
APPLICATION
Standard ELISA test, Western Blot.
ENDOTOXIN LEVEL
<0.2 EU/ug