Human Native Histones

Human Native Histones

★ Download Datasheet PDF ★

 

Origin:

Native

Cat. No.:

41A035

Tag:

No tag

Size:

0.1 mg

Source:

HEK293 cells

Purity:

>90%

Species:

Human

 

 

 

  • INTRODUCTION

    Histones are proteins that package DNA into nucleosomes and are responsible for maintaining the shape and structure of a nucleosome. 

    There are five families of histones known to date, H1/H5, H2A, H2B, H3, and H4. H2A, along with H2B, H3 and H4, is considered as a core histone. 

    Anti-histone autoantibodies are found in 50%-70% of patients with systemic lupus erythematosus (SLE) and in more than 95% of patients with drug-induced lupus erythematosus.

  • DATA SHEET

  • IMMUNOLOGICAL FUNCTION

    As an autoantigen, histones binds with IgG-type human auto-antibodies.

  • APPLICATIONS

    Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.

  • FORMULATION

    Liquid in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, Protease inhibitor, pH8.0).

  • DESCRIPTION

    Native histones separated from HEK293 cells, including H1, H2A, H2B, H3 and H4.

    Antigen for research use or manufacturing only.

  • STORAGE

    Store at –80°C. Avoid repeated freezing /thawing cycles.

  • QUALITY CONTROL TEST

    BCA to determine quantity of the protein.

    SDS PAGE to determine purity of the protein.

  • SDS-PAGE Gel

    Refer to picture 

Quantity

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