Human Native Histones
Histones are proteins that package DNA into nucleosomes and are responsible for maintaining the shape and structure of a nucleosome.
There are five families of histones known to date, H1/H5, H2A, H2B, H3, and H4. H2A, along with H2B, H3 and H4, is considered as a core histone.
Anti-histone autoantibodies are found in 50%-70% of patients with systemic lupus erythematosus (SLE) and in more than 95% of patients with drug-induced lupus erythematosus.
As an autoantigen, histones binds with IgG-type human auto-antibodies.
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.
Lyophilized in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, PH8.0).
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let thelyophilized pellet dissolve completely.
Native histones separated from HEK293 cells, including H1, H2A, H2B, H3 and H4.
Antigen for research use or manufacturing only.
Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C. Avoid repeatedfreezing /thawing cycles.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Refer to picture