Human Native Histones
Histones are proteins that package DNA into nucleosomes and are responsible for maintaining the shape and structure of a nucleosome.
There are five families of histones known to date, H1/H5, H2A, H2B, H3, and H4. H2A, along with H2B, H3 and H4, is considered as a core histone.
Anti-histone autoantibodies are found in 50%-70% of patients with systemic lupus erythematosus (SLE) and in more than 95% of patients with drug-induced lupus erythematosus.
As an autoantigen, histones binds with IgG-type human auto-antibodies.
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.
Liquid in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, Protease inhibitor, pH8.0).
Native histones separated from HEK293 cells, including H1, H2A, H2B, H3 and H4.
Antigen for research use or manufacturing only.
Store at –80°C. Avoid repeated freezing /thawing cycles.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.