Human neutrophil elastase (NE) immunoassay kit
Neutrophil elastase (NE), also known as leukocyte elastase, serine elastase, and elaszym, is one of the hematopoietic serine proteases localized in the primary granules of polymorphonuclear neutrophils (PMNs)1. The primary function of NE is recognized as to participate in the clearance of invaded pathogens through its intracellular and extracellular killing as well as antimicrobial activity, and the degradation of extracellular matrix components, including elastin, collagens, fibroncetin and proteoglycans2.
PRINCIPLE OF THE ASSAY
This assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a polyclonal antibody specific for human NE. Standards and samples are pipetted into the wells and any human NE present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase (HRP)-linked polyclonal antibody specific for human NE is added to the wells. After a final wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human NE bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human NE, the unknown sample concentration can be interpolated from a reference curve included in each assay.
A. Typical representation of standard curve
The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.
Human NE (ng/ml)