PM20D1 is a bidirectional N-fatty-acyl amino acid synthase/hydrolase that regulates the production of N-fatty-acyl amino acids. These metabolites are endogenous chemical uncouplers of mitochondrial respiration. In an UCP1-independent manner, maybe through interaction with mitochondrial transporters, they promote proton leakage into the mitochondrial matrix. PM20D1 may indirectly regulate the bodily dissipation of chemical energy as heat through thermogenic respiration.
Expressed in E.coli with total 521 AA. Mw: 58.1 kDa (calculated).
N-terminal 6xHis-tag, EK recognition site and TEV cleavage site, 44 extra AA (highlighted).
Recombinant antigen for research use or manufacturing only.
AMINO ACID SEQUENCE
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSENLY FQGAMGPRSG EHQRASRIPSQFSKEERVAM KEALKGAIQI PTVTFSSEKS NTTALAEFGK YIHKVFPTVV STSFIQHEVVEEYSHLFTIQ GSDPSLQPYL LMAHFDVVPA PEEGWEVPPF SGLERDGIIYGRGTLDDKNSVMALLQALEL LLIRKYIPRR SFFISLGHDE ESSGTGAQRI SALLQSRGVQ LAFIVDEGGFILDDFIPNFK KPIALIAVSE KGSMNLMLQV NMTSGHSSAP PKETSIGILA AAVSRLEQTP
MPIIFGSGTV VTVLQQLANE FPFPVNIILS NPWLFEPLIS RFMERNPLTN AIIRTTTALTIFKAGVKFNV IPPVAQATVN FRIHPGQTVQ EVLELTKNIVADNRVQFHVL SAFDPLPVSPSDDKALGYQL LRQTVQSVFP EVNITAPVTS IGNTDSRFFT NLTTGIYRFY PIYIQPEDFK
RIHGVNEKIS VQAYETQVKF IFELIQNADT DQEPVSHLHK L
Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C. Avoid repeated freezing/thawing cycles.
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Lyophilized at 1 mg/mL in NaCl 500mM, KCl 2.7mM, Na2HPO4 10mM, KH2PO4 1.8mM, pH 8.0.
ELISA and Western Blotting.
<0.01 EU per 1 μg of the protein by the LAL method.