Human Recombinant Leptin protein
As one of the most important adipose derived hormones, Human Leptin is secreted primarily in the adipocytes of white adipose tissue, and the level of circulating leptin is directly proportional to the total amount of fat in the body. It acts on receptors in the hypothalamus of the brain where it inhibits appetite and has been shown to reduce body weight, food consumption, and plasma glucose levels in various in vivo models.
Recombinant human Leptin without Signal peptide (AA22-167), total 147 amino acids (AA). N-terminal His-tag removed. Mw: 16.1 kDa (calculated). 1 extra AA left (In bold).
AMINO ACID SEQUENCE
AVPIQKVQDDTKTLIKTIVTRINDISHTQSVSSKQKVTGLDFIPGLHPILTLSKMDQTLAVYQQILTSMPSRNVIQISNDLENLRDLLHVLAFSKSCHLPWASG LETLDSLGGVLEASGYSTEVVALSRLQGSLQDM LWQLDLSPGC
Store at –20°C. Avoid repeated freezing/thawing cycles.
Lyophilized in 1 mg/mL in PBS.
Cell culture, animal studies, ELISA and Western blotting.
Add sterile deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.