Human Recombinant Leptin protein
Origin:
Recombinant
Cat. No.:
41B029
Tag:
Tagless
Size:
0.1 mg
Source:
E.coli
Purity:
>99%
Bioactive:
Yes
Species:
Human
INTRODUCTION
As one of the most important adipose derived hormones, Human Leptin is secreted primarily in the adipocytes of white adipose tissue, and the level of circulating leptin is directly proportional to the total amount of fat in the body. It acts on receptors in the hypothalamus of the brain where it inhibits appetite and has been shown to reduce body weight, food consumption, and plasma glucose levels in various in vivo models.
DESCRIPTION
Recombinant human Leptin without Signal peptide (AA22-167), total 147 amino acids (AA). N-terminal His-tag removed. Mw: 16.1 kDa (calculated). 1 extra AA left (In bold).
AMINO ACID SEQUENCE
AVPIQKVQDDTKTLIKTIVTRINDISHTQSVSSKQKVTGLDFIPGLHPILTLSKMDQTLAVYQQILTSMPSRNVIQISNDLENLRDLLHVLAFSKSCHLPWASG LETLDSLGGVLEASGYSTEVVALSRLQGSLQDM LWQLDLSPGC
STORAGE
Store at –20°C. Avoid repeated freezing/thawing cycles.
ENDOTOXIN LEVEL
<0.2 EU/ug.
FORMULATION
Lyophilized in 1 mg/mL in PBS.
APPLICATION
Cell culture, animal studies, ELISA and Western blotting.
RECONSTITUTION
Add sterile deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.