Human Thrombospondin-2 (TSP-2) Elisa kit

Human Thrombospondin-2 (TSP-2) Elisa kit

SKU: 31101

 

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Assay range :  0.156 -10 ng/ml

Size:                  96T/Kit

 

  • INTRODUCTION

    Thrombospondin-2 (TSP-2) is a 150 kDa calcium-binding protein.  TSP-2 inhibits angiogenesis through direct effect on endothelial cell migration, proliferation, survival and apoptosis.  It is a potential risk factor in heart disease.

  • PRINCIPLE OF THE ASSAY

    This assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a monoclonal antibody specific for human TSP-2. Standards and samples are pipetted into the wells and any human TSP-2 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human TSP-2 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human TSP-2 bound initially.  The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human TSP-2, the unknown sample concentration can be interpolated from a reference curve included in each assay.

  • ASSAY PERFORMANCE

    A. Typical representation of standard curve

    The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.  

    Human TSP-2 (ng/ml)

    Absorbance 

    (450 nm)

    Blanked Absorbance

    0

    0.094

    0

    0.156

    0.133

    0.039

    0.312

    0.187

    0.093

    0.625

    0.293

    0.199

    1.25

    0.474

    0.38

    2.5

    0.812

    0.718

    5

    1.317

    1.223

    10

    2.096

    2.002

     

    B. Sensitivity

    The lowest insulin level that can be measured by this assay is 0.156 ng/ml.

     

    C. Precision

    Intra-assay Precision (Precision within an assay) C.V <10%.

    Inter-assay Precision (Precision between assays) C.V <10%.

$400.00Price

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