Human Thrombospondin-2 (TSP-2) ELISA Kit
Thrombospondin-2 (TSP-2) is a 150 kDa calcium-binding protein. TSP-2 inhibits angiogenesis through direct effect on endothelial cell migration, proliferation, survival and apoptosis. It is a potential risk factor in heart disease.
PRINCIPLE OF THE ASSAY
This assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a monoclonal antibody specific for human TSP-2. Standards and samples are pipetted into the wells and any human TSP-2 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human TSP-2 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human TSP-2 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human TSP-2, the unknown sample concentration can be interpolated from a reference curve included in each assay.
A. Typical representation of standard curve
The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.
Human TSP-2 (ng/ml)
The lowest insulin level that can be measured by this assay is 0.156 ng/ml.
Intra-assay Precision (Precision within an assay) C.V <10%.
Inter-assay Precision (Precision between assays) C.V <10%.