• Human Thrombospondin-2 (TSP-2) Elisa kit

    SKU: 31101

    Assay range :  0.156 -10 ng/ml

    Sample size:   100 ul

    Size:                  96T/Kit


    The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.


      Thrombospondin-2 (TSP-2) is a 150 kDa calcium-binding protein.  TSP-2 inhibits angiogenesis through direct effect on endothelial cell migration, proliferation, survival and apoptosis.  It is a potential risk factor in heart disease.


      This assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a monoclonal antibody specific for human TSP-2. Standards and samples are pipetted into the wells and any human TSP-2 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human TSP-2 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human TSP-2 bound initially.  The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human TSP-2, the unknown sample concentration can be interpolated from a reference curve included in each assay.



      A. Sensitivity

      The lowest insulin level that can be measured by this assay is 0.156 ng/ml.


      B. Precision

      Intra-assay Precision (Precision within an assay) C.V <10%.

      Inter-assay Precision (Precision between assays) C.V <10%.


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